Application | Comment | Organism |
---|---|---|
pharmacology | absence of mannose-6-phosphate isomerase causes cell lysis and thus the enzyme is a potential target for inhibition and may be a route to antifungal drugs | Candida albicans |
Cloned (Comment) | Organism |
---|---|
wild-type and selenomethionine-labelled enzyme expressed in Escherichia coli | Candida albicans |
Crystallization (Comment) | Organism |
---|---|
- |
Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Candida albicans | |
Hg2+ | recombinant wild-type enzyme and selenomethionine-labelled enzyme | Candida albicans | |
Zn2+ | - |
Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
mannose 6-phosphate | Hepes buffer | Candida albicans | |
0.8 | - |
mannose 6-phosphate | selenomethionine-labelled enzyme, Hepes buffer | Candida albicans | |
1 | - |
mannose 6-phosphate | Tris/HCl buffer | Candida albicans | |
4 | - |
mannose 6-phosphate | selenomethionine-labelled enzyme, Tris/HCl buffer | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48740 | - |
wild-type recombinant enzyme, electrospray mass spectroscopy | Candida albicans |
49060 | - |
selenomethionine-labelled enzyme expressed in E. coli, electrospray mass spectroscopy | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
wild-type and selenomethionine-labelled enzyme expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
normal and selenomethionine-labelled enzyme expressed in Escherichia coli | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | - |
Candida albicans | D-Fructose 6-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
121 | - |
mannose 6-phosphate | selenomethionine-labelled enzyme | Candida albicans | |
162 | - |
mannose 6-phosphate | wild-type enzyme | Candida albicans |