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Literature summary for 5.3.1.8 extracted from

  • Wells, T.N.C.; Scully, P.; Paravicini, G.; Proudfoot, A.E.I.; Payton, M.A.
    Mechanism of irreversible inactivation of phosphomannose isomerase by silver ions and flamazine (1995), Biochemistry, 34, 7896-7903.
    View publication on PubMed
Search for more literature for 5.3.1.8

Protein Variants

Protein Variants Comment Organism
C150A mutant Cys150Ala shows similar Km-values and maximal velocity as compared to the wild-type enzyme. The mutant enzyme shows no inhibition by silver sulfadiazine, and is 1000fold less sensitive to Hg2+ inhibition Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
Ag+ irreversible inhibition in a two-step process, mannose 6-phosphate protects against inactivation. Mutant enzyme Cys150Ala shows 1000fold less sensitivity than the wild-type enzyme Candida albicans
Silver sulfadiazine wild-type enzyme is inhibited, mutant enzyme Cys150Ala is not inhibited Candida albicans
Silver sulfadiazine no inhibition Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Candida albicans enzyme is involved in biosynthesis of mannan components of the cell walls ?
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Mannose 6-phosphate
-
 Escherichia coli D-Fructose 6-phosphate
-
 ?
D-Mannose 6-phosphate
-
 Candida albicans D-Fructose 6-phosphate
-
 ?
additional information enzyme is involved in biosynthesis of mannan components of the cell walls Candida albicans ?
-
 ?