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Literature summary for 5.3.1.8 extracted from

  • Murata, T.
    Studies on the phosphomannose isomerase of Amorphophallus konjac C. Koch. I. Its isolation and some enzymic properties (1975), Plant Cell Physiol., 16, 953-961.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Amorphophallus konjac
2,2'-dipyridyl
-
Amorphophallus konjac
EDTA
-
Amorphophallus konjac

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
mannose 6-phosphate
-
Amorphophallus konjac

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Amorphophallus konjac

Organism

Organism UniProt Comment Textmining
Amorphophallus konjac
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Amorphophallus konjac

Source Tissue

Source Tissue Comment Organism Textmining
corm
-
Amorphophallus konjac
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.95
-
-
Amorphophallus konjac

Storage Stability

Storage Stability Organism
-20°C, unstable Amorphophallus konjac

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Mannose 6-phosphate
-
Amorphophallus konjac D-Fructose 6-phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 5.5, 1 h, about 35% loss of activity Amorphophallus konjac
40
-
pH 10, about 25% loss of activity Amorphophallus konjac
40
-
pH 6.0-9.5, 1 h, no loss of activity Amorphophallus konjac

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Amorphophallus konjac

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 about 50% of maximal activity at pH 5.5 and pH 9.0 Amorphophallus konjac

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
40°C, 1 h, about 35% loss of activity Amorphophallus konjac
6 9.5 40°C, 1 h, no loss of activity Amorphophallus konjac
10
-
40°C, 1 h, about 25% loss of activity Amorphophallus konjac