Cloned (Comment) | Organism |
---|---|
- |
Clostridioides difficile |
- |
Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by EDTA | Clostridioides difficile | |
additional information | not inhibited by EDTA | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | 20 | L-talose | pH 7.5 and 40°C | Clostridioides difficile | |
30 | - |
D-ribose 5-phosphate | pH 7.5 and 40°C | Clostridioides difficile | |
37 | - |
D-ribose 5-phosphate | pH 8.0 and 70°C | Thermotoga maritima | |
110 | - |
D-ribose | pH 8.0 and 70°C | Thermotoga maritima | |
130 | - |
D-allose | pH 8.0 and 70°C | Thermotoga maritima | |
190 | - |
L-talose | pH 8.0 and 70°C | Thermotoga maritima | |
270 | - |
D-ribose | pH 7.5 and 40°C | Clostridioides difficile | |
460 | - |
D-allose | pH 7.5 and 40°C | Clostridioides difficile |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by metal ions | Clostridioides difficile | |
additional information | not effected by metal ions | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34400 | - |
gel filtration | Clostridioides difficile |
67000 | - |
gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | AM180355 | - |
- |
Clostridioides difficile ATCC BAA-1382D-5 | AM180355 | - |
- |
Thermotoga maritima | Q9X0G9 | - |
- |
Thermotoga maritima DSM 3109 | Q9X0G9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile | D-psicose | - |
? | |
D-allose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima | D-psicose | - |
? | |
D-allose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile ATCC BAA-1382D-5 | D-psicose | - |
? | |
D-allose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima DSM 3109 | D-psicose | - |
? | |
D-gulose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile | D-sorbose | - |
? | |
D-ribose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile | D-ribulose | - |
? | |
D-ribose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima | D-ribulose | - |
? | |
D-ribose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile ATCC BAA-1382D-5 | D-ribulose | - |
? | |
D-ribose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima DSM 3109 | D-ribulose | - |
? | |
D-ribose 5-phosphate | - |
Clostridioides difficile | D-ribulose 5-phosphate | - |
? | |
D-ribose 5-phosphate | - |
Thermotoga maritima | D-ribulose 5-phosphate | - |
? | |
D-ribose 5-phosphate | - |
Thermotoga maritima DSM 3109 | D-ribulose 5-phosphate | - |
? | |
D-talose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima | D-tagatose | - |
? | |
L-allose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima | L-psicose | - |
? | |
L-lyxose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile | L-xylulose | - |
? | |
L-lyxose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima | L-xylulose | - |
? | |
L-lyxose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile ATCC BAA-1382D-5 | L-xylulose | - |
? | |
L-lyxose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima DSM 3109 | L-xylulose | - |
? | |
L-Mannose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile | L-Fructose | - |
? | |
L-Mannose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile ATCC BAA-1382D-5 | L-Fructose | - |
? | |
L-ribose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima | L-ribulose | - |
? | |
L-talose | the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile | L-tagatose | - |
? | |
L-talose | the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose | Thermotoga maritima | L-tagatose | - |
? | |
additional information | the enzyme displays activity only with aldose substrates having hydroxyl groups configured in the same direction at the C2, C3, and C4 positions | Thermotoga maritima | ? | - |
? | |
additional information | the enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose | Clostridioides difficile | ? | - |
? | |
additional information | the enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose | Clostridioides difficile ATCC BAA-1382D-5 | ? | - |
? | |
additional information | the enzyme displays activity only with aldose substrates having hydroxyl groups configured in the same direction at the C2, C3, and C4 positions | Thermotoga maritima DSM 3109 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 17200, SDS-PAGE | Clostridioides difficile |
tetramer | 2 * 16700, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
ribose-5-phosphate isomerase | - |
Clostridioides difficile |
ribose-5-phosphate isomerase | - |
Thermotoga maritima |
RpiB | - |
Clostridioides difficile |
RpiB | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
substrate: D-ribose | Clostridioides difficile |
70 | - |
substrate: L-talose | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life: 53 min | Clostridioides difficile |
75 | - |
half-life: 191 min | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
140 | - |
L-talose | pH 7.5 and 40°C | Clostridioides difficile | |
140 | - |
D-allose | pH 8.0 and 70°C | Thermotoga maritima | |
160 | - |
D-ribose | pH 7.5 and 40°C | Clostridioides difficile | |
200 | - |
D-allose | pH 7.5 and 40°C | Clostridioides difficile | |
500 | - |
D-ribose | pH 8.0 and 70°C | Thermotoga maritima | |
1340 | - |
L-talose | pH 8.0 and 70°C | Thermotoga maritima | |
15100 | - |
D-ribose 5-phosphate | pH 7.5 and 40°C | Clostridioides difficile | |
20000 | - |
D-ribose 5-phosphate | pH 8.0 and 70°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
substrate: D-ribose | Clostridioides difficile |
8 | - |
substrate: L-talose | Thermotoga maritima |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Clostridioides difficile | - |
- |
6.4 |
Thermotoga maritima | - |
- |
7.2 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
L-talose | pH 7.5 and 40°C | Clostridioides difficile | |
0.4 | - |
D-allose | pH 7.5 and 40°C | Clostridioides difficile | |
0.6 | - |
D-ribose | pH 7.5 and 40°C | Clostridioides difficile | |
1.1 | - |
D-allose | pH 8.0 and 70°C | Thermotoga maritima | |
4.7 | - |
D-ribose | pH 8.0 and 70°C | Thermotoga maritima | |
7 | - |
L-talose | pH 8.0 and 70°C | Thermotoga maritima | |
500 | - |
D-ribose 5-phosphate | pH 7.5 and 40°C | Clostridioides difficile | |
540 | - |
D-ribose 5-phosphate | pH 8.0 and 70°C | Thermotoga maritima |