Cloned (Comment) | Organism |
---|---|
expression of MJ1603 in Escherichia coli strain Rosetta (DE3) | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant MJ1603, microbatch-under-oil method, 0.0005 ml of 8.1 mg/ml protein in 20 mM Tris-HCl buffer, pH 8.0, containing 200 mM NaCl are mixed with 0.0005 ml of crystallization reagent, consisting of 0.1 M acetate, pH 4.5 containing 40% v/v 1,2-propanediol and 0.05 M calcium acetate, 18°C. The mixture is covered with 0.015 ml silicone and paraffin oil, X-ray diffraction structure determination and analysis at 1.78 A resolution, molecular replacement and modeling | Methanocaldococcus jannaschii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate | Methanocaldococcus jannaschii | - |
D-ribulose 5-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58998 | strain DSM 2661 | - |
Purification (Comment) | Organism |
---|---|
recombinant MJ1603 from Escherichia coli strain Rosetta (DE3) by anion exchange and hydroxyapatite chromatography, and gel filtration | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate | - |
Methanocaldococcus jannaschii | D-ribulose 5-phosphate | - |
r | |
D-ribose 5-phosphate | the open configuration of ribose 5-phosphate is required for isomerization and is reversibly converted to ribulose 5-phosphate | Methanocaldococcus jannaschii | D-ribulose 5-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | RpiA crystal structure, overview | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
MJ1603 | - |
Methanocaldococcus jannaschii |
RpiA | - |
Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
metabolism | two evolutionary distinct forms of the enzyme, RpiA and RpiB, with different amino acid sequences and molecular weights exist, that both catalyze the reversible conversion of ribose 5-phosphate to ribulose 5-phosphate. RpiA is found in the bacterial, plant and animal kingdoms, whereas RpiB is less widespread and is found in bacterial sources | Methanocaldococcus jannaschii |