Literature summary for 5.3.1.5 extracted from

Dmytruk, O.V.; Voronovsky, A.Y.; Abbas, C.A.; Dmytruk, K.V.; Ishchuk, O.P.; Sibirny, A.A.
Overexpression of bacterial xylose isomerase and yeast host xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha (2008), FEMS Yeast Res., 8, 165-173.

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Application

Application	Comment	Organism
energy production	genetic engineering of the yeast Hansenula polymorpha in order to increase ethanol production by D-xylose fermentation	Escherichia coli
synthesis	co-overexpression of xylose isomerase and endogenous xylulokinase in a Hansenula polymorpha strain lacking NAD(P)H-dependent xylose reductase and NAD-dependent xylitol dehydrogenases activities. Recombinant strain displays improved ethanol production during the fermentation of xylose	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
co-overexpression of genes encoding xylose isomerase (EcxylA, Escherichia coli) and xylulokinase (XYL3, Hansenula polymorpha), expression of both genes is driven by Hansenula polymorpha GAP-DH promoter	Escherichia coli
expression in Hansenula polymorpha	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
DELTAxyl1	deficient in xylose reductase	Escherichia coli
DELTAxyl1 DELTAxyl2-A	deficient in xylose reductase and xylitol dehydrogenase xyl2-A	Escherichia coli
DELTAxyl1 DELTAxyl2-A (EcxylA) No. 1	deficient in xylose reductase and xylitol dehydrogenase xyl2-A, transformed with xylose isomerase xylA from Escherichia coli, strain 1	Escherichia coli
DELTAxyl1 DELTAxyl2-A (EcxylA) No. 2	deficient in xylose reductase and xylitol dehydrogenase xyl2-A, transformed with xylose isomerase xylA from Escherichia coli, strain 2	Escherichia coli
DELTAxyl1 DELTAxyl2-A (EcxylA) No. 4L/3	deficient in xylose reductase and xylitol dehydrogenase xyl2-A, transformed with xylose isomerase xylA from Escherichia coli, strain 4L/3	Escherichia coli
DELTAxyl1 DELTAxyl2-A DELTAxyl2-B	deficient in xylose reductase and xylitol dehydrogenases xyl2-A and xyl2-B	Escherichia coli
DELTAxyl1 DELTAxyl2-A DELTAxyl2-B (EcxylA HpXYL3)	deficient in xylose reductase and xylitol dehydrogenases xyl2-A and xyl2-B, transformed with xylose isomerase xylA from Escherichia coli and overexpressing endogenous xylulukinase xyl3	Escherichia coli
DELTAxyl1 DELTAxyl2-A DELTAxyl2-B (EcxylA) No. 1	deficient in xylose reductase and xylitol dehydrogenases xyl2-A and xyl2-B, transformed with xylose isomerase xylA from Escherichia coli, strain 1	Escherichia coli
DELTAxyl1 DELTAxyl2-A DELTAxyl2-B (EcxylA) No. 2	deficient in xylose reductase and xylitol dehydrogenases xyl2-A and xyl2-B, transformed with xylose isomerase xylA from Escherichia coli, strain 2	Escherichia coli
Hansenula polymorpha	yeast, strain CBS4732s leu2-2, deficient in beta-isopropyl malate dehydrogenase, deletions of genes encoding xylose reductase (xyl1) and xylitol dehydrogenases (xyl2-A and xyl2-B) - overexpression of xylA gene (Escherichia coli) and endogenous xyl3 gene	Escherichia coli
additional information	co-overexpression of xylose isomerase and endogenous xylulokinase in a Hansenula polymorpha strain lacking NAD(P)H-dependent xylose reductase and NAD-dependent xylitol dehydrogenases activities. Recombinant strain displays improved ethanol production during the fermentation of xylose	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli	-	strain DH5alpha	-
Escherichia coli DH5-alpha	-	strain DH5alpha	-
no activity in Hansenula polymorpha	-	-	-
no activity in Hansenula polymorpha CBS4732s leu2-2	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	measurement of xylose consumption and ethanol production	Escherichia coli
additional information	-	modification of established spectrophotometrical activity assay	Escherichia coli
additional information	-	no activity in: CBS 4732s leu2-2(control strain), DELTAxyl1, DELTAxyl1 DELTAxyl2-A, DELTAxyl1 DELTAxyl2-A DELTAxyl2-B	Escherichia coli
0.042	-	+/-0.003, DELTAxyl1 DELTAxyl2-A(EcxylA) No. 1	Escherichia coli
0.047	-	+/-0.003, DELTAxyl1 DELTAxyl2-A(EcxylA) No. 2	Escherichia coli
0.05	-	+/-0.004, DELTAxyl1 DELTAxyl2-A(EcxylA) No. 4L/3	Escherichia coli
0.151	-	+/-0.009, DELTAxyl1 DELTAxyl2-A DELTAxyl2-B (EcxylA HpXYL3)	Escherichia coli
0.152	-	+/-0.009, DELTAxyl1 DELTAxyl2-A DELTAxyl2-B (EcxylA) No. 1	Escherichia coli
0.188	-	+/-0.010, DELTAxyl1 DELTAxyl2-A DELTAxyl2-B (EcxylA) No. 2	Escherichia coli
0.209	-	+/-0.011, Escherichia coli, control strain	Escherichia coli

Synonyms

Synonyms	Comment	Organism
xylose isomerase	-	Escherichia coli
xylose isomerase	-	no activity in Hansenula polymorpha

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Release 2023.1 (January 2023)