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Literature summary for 5.3.1.5 extracted from
- Biosynthetic features and properties of xylose isomerases from Arthrobacter nicotianae, Escherichia coli, and Erwinia carotovora subsp. atroseptica (2006), Appl. Biochem. Microbiol., 42, 246-251.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | if D-xylose is the carbon source D-glucose supplementation stimulates formation of xylose isomerase | Glutamicibacter nicotianae |  |
| sucrose | - |
Glutamicibacter nicotianae | |
| xylitol | - |
Escherichia coli | |
| xylitol | - |
Glutamicibacter nicotianae | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | suppressed enzyme synthesis by increased content from 0.1 to 1.5%, productivity maxima at 0.1%, if D-xylose is the carbon source: D-glucose supplementation suppresses formation of xylose isomerase | Escherichia coli | |
| D-glucose | suppressed enzyme synthesis by increased D-glucose content from 0.1 to 1.5%, productivity maxima at 1.0% | Glutamicibacter nicotianae | |
| D-glucose | suppressed enzyme synthesis by increased D-glucose content from 0.1 to 1.5%, productivity maxima at 0.1% D-glucose, if D-xylose is the carbon source D-glucose supplementation suppresses formation of xylose isomerase | Pectobacterium atrosepticum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Xylose | Escherichia coli | - |
D-Xylulose | - |
r | |
| D-Xylose | Glutamicibacter nicotianae | - |
D-Xylulose | - |
r | |
| D-Xylose | Pectobacterium atrosepticum | - |
D-Xylulose | - |
r | |
| D-Xylose | Glutamicibacter nicotianae BIM B-5 | - |
D-Xylulose | - |
r | |
| D-Xylose | Escherichia coli HB101xylA | - |
D-Xylulose | - |
r | |
| D-Xylose | Pectobacterium atrosepticum jn42xylA | - |
D-Xylulose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli HB101xylA | - |
- |
- |
| Glutamicibacter nicotianae | - |
- |
- |
| Glutamicibacter nicotianae BIM B-5 | - |
- |
- |
| Pectobacterium atrosepticum | - |
- |
- |
| Pectobacterium atrosepticum jn42xylA | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Arthrobacter nicotianae and Erwinia carotovora subsp. atroseptica | Escherichia coli |
| PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Escherichia coli and Erwinia carotovora subsp. atroseptica | Glutamicibacter nicotianae |
| PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from from Arthrobacter nicotianae and Escherichia coli | Pectobacterium atrosepticum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.22 | - |
enzyme production in the presence of D-glucose | Escherichia coli |
| 0.46 | - |
crude extract | Pectobacterium atrosepticum |
| 0.46 | - |
enzyme production in the presence of D-xylose | Escherichia coli |
| 0.47 | - |
enzyme production in the presence of D-xylose | Glutamicibacter nicotianae |
| 0.71 | - |
enzyme production in the presence of D-xylose | Pectobacterium atrosepticum |
| 0.73 | - |
enzyme production in the presence of sucrose | Glutamicibacter nicotianae |
| 0.81 | - |
enzyme production in the presence of D-glucose | Pectobacterium atrosepticum |
| 1.39 | - |
enzyme production in the presence of xylitol | Escherichia coli |
| 1.63 | - |
crude extract | Glutamicibacter nicotianae |
| 1.69 | - |
crude extract | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose | lower efficiency | Escherichia coli | ? | - |
? | |
| D-glucose | lower efficiency | Glutamicibacter nicotianae | ? | - |
? | |
| D-glucose | lower efficiency | Pectobacterium atrosepticum | ? | - |
? | |
| D-glucose | lower efficiency | Glutamicibacter nicotianae BIM B-5 | ? | - |
? | |
| D-glucose | lower efficiency | Escherichia coli HB101xylA | ? | - |
? | |
| D-glucose | lower efficiency | Pectobacterium atrosepticum jn42xylA | ? | - |
? | |
| D-Ribose | weak effects | Escherichia coli | ? | - |
? | |
| D-Ribose | weak effects | Glutamicibacter nicotianae | ? | - |
? | |
| D-Ribose | weak effects | Pectobacterium atrosepticum | ? | - |
? | |
| D-Ribose | weak effects | Glutamicibacter nicotianae BIM B-5 | ? | - |
? | |
| D-Ribose | weak effects | Escherichia coli HB101xylA | ? | - |
? | |
| D-Ribose | weak effects | Pectobacterium atrosepticum jn42xylA | ? | - |
? | |
| D-Xylose | - |
Escherichia coli | D-Xylulose | - |
r | |
| D-Xylose | - |
Glutamicibacter nicotianae | D-Xylulose | - |
r | |
| D-Xylose | - |
Pectobacterium atrosepticum | D-Xylulose | - |
r | |
| D-Xylose | - |
Glutamicibacter nicotianae BIM B-5 | D-Xylulose | - |
r | |
| D-Xylose | - |
Escherichia coli HB101xylA | D-Xylulose | - |
r | |
| D-Xylose | - |
Pectobacterium atrosepticum jn42xylA | D-Xylulose | - |
r | |
| L-Arabinose | weak effects | Escherichia coli | ? | - |
? | |
| L-Arabinose | weak effects | Glutamicibacter nicotianae | ? | - |
? | |
| L-Arabinose | weak effects | Pectobacterium atrosepticum | ? | - |
? | |
| L-Arabinose | weak effects | Glutamicibacter nicotianae BIM B-5 | ? | - |
? | |
| L-Arabinose | weak effects | Escherichia coli HB101xylA | ? | - |
? | |
| L-Arabinose | weak effects | Pectobacterium atrosepticum jn42xylA | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-xylose: ketol-isomerase | - |
Escherichia coli |
| D-xylose: ketol-isomerase | - |
Glutamicibacter nicotianae |
| D-xylose: ketol-isomerase | - |
Pectobacterium atrosepticum |
| xylose isomerase | - |
Escherichia coli |
| xylose isomerase | - |
Glutamicibacter nicotianae |
| xylose isomerase | - |
Pectobacterium atrosepticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Escherichia coli |
| 70 | - |
- |
Glutamicibacter nicotianae |
| 75 | - |
- |
Pectobacterium atrosepticum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
pH 7.5, 2 h | Escherichia coli |
| 70 | - |
pH 7.5, 2 h | Glutamicibacter nicotianae |
| 70 | 80 | pH 7.8, 2 h, after following 2 h incubation at 80°C 37.6% loss of activity, 70-75% loss of activity at 80°C | Pectobacterium atrosepticum |
| 80 | - |
2 h leads to 30.1% loss of activity | Escherichia coli |
| 80 | - |
2 h leads to 30.5% loss of activity | Glutamicibacter nicotianae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
| 7.5 | - |
- |
Glutamicibacter nicotianae |
| 8 | - |
- |
Pectobacterium atrosepticum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | 70°C for 1 h, 70-75% loss of activity at 80°C | Escherichia coli |
| 7 | 8 | 70°C for 1 h, 70-75% loss of activity at 80°C | Glutamicibacter nicotianae |
| 7 | 8 | 70°C for 1 h, 70-75% loss of activity at 80°C | Pectobacterium atrosepticum |
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