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Literature summary for 5.3.1.5 extracted from
- Influence of divalent cations on the structural thermostability and thermal inactivation kinetics of class II xylose isomerases (2005), FEBS J., 272, 1454-1464.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Thermotoga neapolitana |
| expression in Escherichia coli HB101 | Bacillus licheniformis |
| expression in Escherichia coli HB101 | Thermoanaerobacterium thermosulfurigenes |
| expression in Escherichia coli JM105 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D309K | no activity, Tm of 95.5°C in the presence of 5 mM Mg2+ and 0.5 mM Co2+ | Thermotoga neapolitana |
| E232K | no activity, Tm of 100.7°C in the presence of 5 mM Mg2+ and 0.5 mM Co2+ | Thermotoga neapolitana |
| E232K/D309K | no activity, Tm of 96.5°C in the presence of 5 mM Mg2+ and 0.5 mM Co2+ | Thermotoga neapolitana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | XI is substantially structurally stabilized in the presence of CO2+ | Escherichia coli |  |
| Co2+ | XI is substantially structurally stabilized in the presence of CO2+ | Bacillus licheniformis | |
| Co2+ | XI is substantially structurally stabilized in the presence of CO2+ | Thermoanaerobacterium thermosulfurigenes | |
| Mg2+ | XI is to a lesser extent structurally stabilized in the presence of Mg2+ | Escherichia coli | |
| Mg2+ | XI is to a lesser extent structurally stabilized in the presence of Mg2+ | Bacillus licheniformis | |
| Mg2+ | XI is to a lesser extent structurally stabilized in the presence of Mg2+ | Thermoanaerobacterium thermosulfurigenes | |
| Mn2+ | XI is substantially structurally stabilized in the presence of Mn2+ | Escherichia coli | |
| Mn2+ | XI is substantially structurally stabilized in the presence of Mn2+ | Bacillus licheniformis | |
| Mn2+ | XI is substantially structurally stabilized in the presence of Mn2+ | Thermoanaerobacterium thermosulfurigenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE | Thermotoga neapolitana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Xylose | Escherichia coli | - |
D-Xylulose | - |
? | |
| D-Xylose | Bacillus licheniformis | - |
D-Xylulose | - |
? | |
| D-Xylose | Thermotoga neapolitana | - |
D-Xylulose | - |
? | |
| D-Xylose | Thermoanaerobacterium thermosulfurigenes | - |
D-Xylulose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Thermoanaerobacterium thermosulfurigenes | - |
- |
- |
| Thermotoga neapolitana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Bacillus licheniformis |
- |
Thermotoga neapolitana |
- |
Thermoanaerobacterium thermosulfurigenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glucose | - |
Escherichia coli | D-Fructose | - |
? | |
| D-Glucose | - |
Bacillus licheniformis | D-Fructose | - |
? | |
| D-Glucose | - |
Thermotoga neapolitana | D-Fructose | - |
? | |
| D-Xylose | - |
Escherichia coli | D-Xylulose | - |
? | |
| D-Xylose | - |
Bacillus licheniformis | D-Xylulose | - |
? | |
| D-Xylose | - |
Thermotoga neapolitana | D-Xylulose | - |
? | |
| D-Xylose | - |
Thermoanaerobacterium thermosulfurigenes | D-Xylulose | - |
? | |
| glucose | - |
Thermoanaerobacterium thermosulfurigenes | fructose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Thermotoga neapolitana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XI | - |
Escherichia coli |
| XI | - |
Bacillus licheniformis |
| XI | - |
Thermotoga neapolitana |
| XI | - |
Thermoanaerobacterium thermosulfurigenes |
| xylose isomerase | - |
Escherichia coli |
| xylose isomerase | - |
Bacillus licheniformis |
| xylose isomerase | - |
Thermotoga neapolitana |
| xylose isomerase | - |
Thermoanaerobacterium thermosulfurigenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Tm: 50.3°C (apoenzyme), 53.3°C (in the presence of 5 microM Mg2+), 73.4°C (in the presence of 5 microM Co2+), 73.6°C (in the presence of 5 microM Mn2+) | Bacillus licheniformis |
| additional information | - |
Tm: 50.8°C (apoenzyme), 56.2°C (in the presence of 5 microM Mg2+), 56.2°C (in the presence of 5 microM Co2+), 57.3°C (in the presence of 5 microM Mn2+) | Escherichia coli |
| additional information | - |
Tm: 64.1°C (apoenzyme), 83.0°C (in the presence of 5 microM Mg2+), 86.0°C (in the presence of 5 microM Co2+), 86.1°C (in the presence of 5 microM Mn2+) | Thermoanaerobacterium thermosulfurigenes |
| additional information | - |
Tm: 96.4°C (apoenzyme), 97.6°C (in the presence of 5 microM Mg2+), 97.5°C (in the presence of 5 microM Co2+), 96.9°C (in the presence of 5 microM Mn2+), 100.4°C (in the presence of 5 mM Mg2+), 100.0°C (in the presence of 5 mM Co2+), 100.5°C (in the presence of 5 mM Mn2+), 100.9°C (in the presence of 5 mM Ni2+), 100.5°C (in the presence of 5 mM Ca2+), half-lives: 83.2 min in the presence of 2 mM Mn2+ at 99°C, 6.9 min in the presence of 2 mM Mn2+ at 102°C, 4.2 min in the presence of 2 mM Mn2+ at 104°C, 2.3 min in the presence of 2 mM Mn2+ at 106°C, 59.5 min in the presence of 2 mM Co2+ at 96°C, 14 min in the presence of 2 mM Co2+ at 99°C, 5.8 min in the presence of 2 mM Co2+ at 102°C, 2.9 min in the presence of 2 mM Co2+ at 104°C, 12 min in the presence of 2 mM Mg2+ at 92°C, 2.3 min in the presence of 2 mM Mg2+ at 96°C, 1.9 min in the presence of 2 mM Mg2+ at 99°C, 1.3 min in the presence of 2 mM Mg2+ at 102°C, 30 min in the absence of metal ions at 87°C, 11.3 min in the absence of metal ions at 90°C, 7.5 min in the presence of 5 mM Mg2+ and 0.5 mM Co2+ at 100°C, 3.0 min in the presence of 0.5 mM Co2+ at 100°C | Thermotoga neapolitana |
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