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Literature summary for 5.3.1.5 extracted from

  • Santa, H.; Kammonen, J.; Lehtonen, O.; Karimaki, J.; Pastinen, O.; Leisola, M.; Turunen, O.
    Stochastic boundary molecular dynamics simulation of L-ribose in the active site of Actinoplanes missouriensis xylose isomerase and its Val135Asn mutant with improved reaction rate (2005), Biochim. Biophys. Acta, 1749, 65-73.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Actinoplanes missouriensis

Protein Variants

Protein Variants Comment Organism
V135N no effect on the reaction with D-xylose and L-arabinose, reaction efficiency with L-ribose is increased 2-4fold, reaction with D-glucose is impaired Actinoplanes missouriensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
244
-
D-glucose mutant V135N Actinoplanes missouriensis
438
-
L-ribose mutant V135N Actinoplanes missouriensis
713
-
D-glucose wild-type Actinoplanes missouriensis
1312
-
L-ribose wild-type Actinoplanes missouriensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Xylose Actinoplanes missouriensis
-
D-Xylulose
-
?

Organism

Organism UniProt Comment Textmining
Actinoplanes missouriensis P12851
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, by heat precipitation and cation exchange, proteins were 95% pure when estimated in SDS-PAGE Actinoplanes missouriensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
Actinoplanes missouriensis D-Fructose
-
?
D-ribose
-
Actinoplanes missouriensis D-ribulose
-
r
D-ribose
-
Actinoplanes missouriensis L-arabinose
-
r
D-Xylose
-
Actinoplanes missouriensis D-Xylulose
-
?
L-arabinose
-
Actinoplanes missouriensis D-ribose
-
r
L-arabinose
-
Actinoplanes missouriensis D-ribulose
-
r
L-ribose
-
Actinoplanes missouriensis L-ribulose
-
?

Synonyms

Synonyms Comment Organism
XI
-
Actinoplanes missouriensis
xylose isomerase
-
Actinoplanes missouriensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0226
-
L-ribose mutant V135N Actinoplanes missouriensis
0.0252
-
L-ribose wild-type Actinoplanes missouriensis
3.47
-
D-glucose mutant V135N Actinoplanes missouriensis
26.3
-
D-glucose wild-type Actinoplanes missouriensis