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Literature summary for 5.3.1.5 extracted from

  • Smith, C.A.; Rangarajan, M.; Hartley, B.S.
    D-Xylose (D-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728 (1991), Biochem. J., 277, 255-261.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.5

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strict competitive against Mg2+ Arthrobacter sp.
D-sorbitol mixed-type inhibition Arthrobacter sp.
D-xylitol competitive Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
 D-xylose D-xylose, 30°C Arthrobacter sp.
86
-
 D-glucose D-lyxose, 30°C Arthrobacter sp.
170
-
 D-fructose 30°C Arthrobacter sp.
190
-
 D-ribose 30°C Arthrobacter sp.
210
-
 D-glucose 60°C Arthrobacter sp.
225
-
 D-glucose 30°C Arthrobacter sp.
250
-
 D-glucose D-fructose, 60°C Arthrobacter sp.
280
-
 L-arabinose 30°C Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ less effective than Mg2+ in D-xylose isomerization Arthrobacter sp.
Mg2+ most effective in activation of D-xylose isomerization Arthrobacter sp.
Mn2+ less effective than Mg2+ in activation of D-xylose isomerization Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 4 * 47000, SDS-PAGE Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.5
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
 Arthrobacter sp. D-Fructose
-
 ?
D-Lyxose
-
 Arthrobacter sp. ?
-
 ?
D-Ribose
-
 Arthrobacter sp. ?
-
 ?
D-Xylose
-
 Arthrobacter sp. D-Xylulose
-
 ?
L-Arabinose poor substrate Arthrobacter sp. ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 47000, SDS-PAGE Arthrobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0617
-
 D-Lyxose 30°C Arthrobacter sp.
0.515
-
 D-ribose 30°C Arthrobacter sp.
1.31
-
 L-arabinose 30°C Arthrobacter sp.
1.67
-
 D-fructose 30°C Arthrobacter sp.
2.32
-
 D-glucose 30°C Arthrobacter sp.
8.88
-
 D-xylose 30°C Arthrobacter sp.
19.9
-
 D-glucose 60°C Arthrobacter sp.
20.5
-
 D-fructose 60°C Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 D-fructose Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 pH 5.5: about 40% of maximal activity, pH 9.5: about 80% of maximal activity, D-fructose isomerization Arthrobacter sp.