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Literature summary for 5.3.1.5 extracted from
- Production and purification of extracellular D-xylose isomerase from an alkaliphilic, thermophilic Bacillus sp. (1994), Appl. Environ. Microbiol., 60, 4495-4499.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inactivation in presence of Mg2+, Co2+ or Mn2+ | Bacillus sp. (in: Bacteria) |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.66 | - |
D-xylose | - |
Bacillus sp. (in: Bacteria) | |
| 142 | - |
D-glucose | - |
Bacillus sp. (in: Bacteria) | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | when the metal ions are added separately the activation is 48-52%. The combination of Mg2+ and Co2+ results in activation of 83%. The combination of Mg2+ and Mn2+ or Co2+ and Mn2+ results in 58-59% activation | Bacillus sp. (in: Bacteria) | |
| Co2+ | Mg2+, Mn2+ or Co2+ required for maximal activity | Bacillus sp. (in: Bacteria) | |
| Mg2+ | when the metal ions are added separately the activation is 48-52%. The combination of Mg2+ and Co2+ results in activation of 83%. The combination of Mg2+ and Mn2+ or Co2+ and Mn2+ results in 58-59% activation | Bacillus sp. (in: Bacteria) | |
| Mg2+ | Mg2+, Mn2+ or Co2+ required for maximal activity | Bacillus sp. (in: Bacteria) | |
| Mn2+ | when the metal ions are added separately the activation is 48-52%. The combination of Mg2+ and Co2+ results in activation of 83%. The combination of Mg2+ and Mn2+ or Co2+ and Mn2+ results in 58-59% activation | Bacillus sp. (in: Bacteria) | |
| Mn2+ | Mg2+, Mn2+ or Co2+ required for maximal activity | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
3 * 50000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 160000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.4 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glucose | - |
Bacillus sp. (in: Bacteria) | D-Fructose | - |
r | |
| D-Xylose | - |
Bacillus sp. (in: Bacteria) | D-Xylulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 50000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | 30 min, in presence of Co2+, stable | Bacillus sp. (in: Bacteria) |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
D-glucose | - |
Bacillus sp. (in: Bacteria) | |
| 230 | - |
D-xylose | - |
Bacillus sp. (in: Bacteria) | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus sp. (in: Bacteria) |
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