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Literature summary for 5.3.1.5 extracted from

  • Liu, S.Y.; Wiegel, J.; Gherardine, F.C.
    Purification and cloning of a thermostable xylose (glucose) isomerase with an acidic pH optimum from Thermoanaerobacterium strain JW/SL-YS 489 (1996), J. Bacteriol., 178, 5938-5945.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoanaerobacterium saccharolyticum

General Stability

General Stability Organism
Mn2+, Co2+, or Mg2+ required for thermal stability Thermoanaerobacterium saccharolyticum

Inhibitors

Inhibitors Comment Organism Structure
EDTA Mn2+, Mg2+, or Co2+ restore 60-70% of the original xylose isomerase activity Thermoanaerobacterium saccharolyticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16
-
 D-xylose
-
 Thermoanaerobacterium saccharolyticum
19
-
 D-xylose
-
 Thermoanaerobacterium saccharolyticum
55
-
 D-fructose
-
 Thermoanaerobacterium saccharolyticum
120
-
 D-glucose
-
 Thermoanaerobacterium saccharolyticum
130
-
 D-glucose
-
 Thermoanaerobacterium saccharolyticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, SDS-PAGE Thermoanaerobacterium saccharolyticum
200000
-
 gel filtration Thermoanaerobacterium saccharolyticum

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium saccharolyticum
-
-
-
Thermoanaerobacterium saccharolyticum
-
 B6A-RI
-
Thermoanaerobacterium saccharolyticum B6A-RI
-
 B6A-RI
-
Thermoanaerobacterium saccharolyticum JW/SL-YS 489
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoanaerobacterium saccharolyticum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.18
-
-
 Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Fructose
-
 Thermoanaerobacterium saccharolyticum ?
-
 ?
D-Fructose
-
 Thermoanaerobacterium saccharolyticum B6A-RI ?
-
 ?
D-Fructose
-
 Thermoanaerobacterium saccharolyticum JW/SL-YS 489 ?
-
 ?
D-Glucose
-
 Thermoanaerobacterium saccharolyticum D-Fructose
-
 ?
D-Glucose
-
 Thermoanaerobacterium saccharolyticum B6A-RI D-Fructose
-
 ?
D-Glucose
-
 Thermoanaerobacterium saccharolyticum JW/SL-YS 489 D-Fructose
-
 ?
D-Xylose
-
 Thermoanaerobacterium saccharolyticum D-Xylulose
-
 ?
D-Xylose
-
 Thermoanaerobacterium saccharolyticum B6A-RI D-Xylulose
-
 ?
D-Xylose
-
 Thermoanaerobacterium saccharolyticum JW/SL-YS 489 D-Xylulose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 85 at pH 6.5 Thermoanaerobacterium saccharolyticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 pH 6.5, stable for more than 5 h Thermoanaerobacterium saccharolyticum
82
-
 half-life: 1 h Thermoanaerobacterium saccharolyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
 60°C Thermoanaerobacterium saccharolyticum
6.8
-
 80°C Thermoanaerobacterium saccharolyticum
7 7.5
-
 Thermoanaerobacterium saccharolyticum