Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | above 5 mM | Streptomyces sp. | |
Ca2+ | - |
Streptomyces sp. | |
D-sorbitol | competitive | Streptomyces sp. | |
D-xylitol | competitive | Streptomyces sp. | |
EDTA | above 5 mM | Streptomyces sp. | |
PCMB | above 5 mM | Streptomyces sp. | |
Sn2+ | - |
Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
D-xylose | - |
Streptomyces sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Streptomyces sp. | |
Mg2+ | activates | Streptomyces sp. | |
Mn2+ | activates | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | 73000 | gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Streptomyces sp. |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.39 | - |
- |
Streptomyces sp. |
Storage Stability | Organism |
---|---|
-15°C, pH 7.5, 5 mM MgSO4, stable for at least 6 months, in absence of Mg2+ loss of activity within 3 weeks | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | no activity | Streptomyces sp. | D-Fructose | - |
? | |
D-Ribose | no activity | Streptomyces sp. | ? | - |
? | |
D-Xylose | - |
Streptomyces sp. | D-Xylulose | - |
? | |
L-Arabinose | no activity | Streptomyces sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, stable up to | Streptomyces sp. |
80 | - |
10 min, 80% loss of activity in absence of metal ions, 50% loss of activity in presence of 1 mM Mg2+, no loss of activity in presence of 1 mM Co2+ | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7: about 40% of maximal activity, pH 10: about 13.5% of maximal activity | Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
highest stability at, 60°C, 30 min | Streptomyces sp. |