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Literature summary for 5.3.1.5 extracted from
- Purification, immobilization, and some properties of glucose isomerase from Streptomyces flavogriseus (1979), Appl. Environ. Microbiol., 38, 1111-1119.
General Stability
| General Stability | Organism |
|---|---|
| after operating at 70°C for 5 days, the remaining enzyme activities of the immobilized enzyme and the whole cells in the presence of Mg2+ and Co2+ is 75% | Streptomyces flavogriseus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Streptomyces flavogriseus |  |
| Cu2+ | - |
Streptomyces flavogriseus | |
| Hg2+ | - |
Streptomyces flavogriseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
D-xylose | soluble enzyme | Streptomyces flavogriseus | |
| 0.104 | - |
D-xylose | immobilized enzyme | Streptomyces flavogriseus | |
| 0.249 | - |
D-glucose | soluble enzyme | Streptomyces flavogriseus | |
| 0.297 | - |
D-glucose | immobilized enzyme | Streptomyces flavogriseus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Mg2+ or Co2+ required for activity | Streptomyces flavogriseus | |
| Mg2+ | Co2+ or Mg2+ required for activity | Streptomyces flavogriseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Streptomyces flavogriseus |
| 171000 | - |
equilibrium sedimentation | Streptomyces flavogriseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces flavogriseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Streptomyces flavogriseus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.2 | - |
- |
Streptomyces flavogriseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glucose | - |
Streptomyces flavogriseus | D-Fructose | - |
? | |
| D-Xylose | - |
Streptomyces flavogriseus | D-Xylulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Streptomyces flavogriseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
soluble and immobilized enzyme | Streptomyces flavogriseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces flavogriseus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | soluble enzyme, stable | Streptomyces flavogriseus |
| 5.5 | 9 | immobilized enzyme stable | Streptomyces flavogriseus |
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