Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris and in Escherichia coli strain BL21(DE3)pLysS | Arthrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 82% residual activity at 10 mM | Arthrobacter sp. | |
additional information | no activity loss is observed after treatment with EDTA for 12 h | Arthrobacter sp. | |
Ni2+ | 70% residual activity at 10 mM | Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
119 | - |
D-galactose | at pH 7.0 and 50°C | Arthrobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not dependent on divalent metal ions, since it is only marginally activated by Mg2+, Mn2+ or Ca2+ | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
6 * 55000, SDS-PAGE | Arthrobacter sp. |
55183 | - |
6 * 55183, calculated from amino acid sequence | Arthrobacter sp. |
335000 | - |
gel filtration | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | Arthrobacter sp. | - |
L-ribulose | - |
? | |
L-arabinose | Arthrobacter sp. 22c | - |
L-ribulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | H6UGW9 | - |
- |
Arthrobacter sp. 22c | H6UGW9 | - |
- |
Purification (Comment) | Organism |
---|---|
Fractogel EMD DEAE chromatography and Fractogel EMD TMAE chromatography | Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.026 | - |
cell extract, at pH 7.5 and 52°C | Arthrobacter sp. |
0.232 | - |
after 8.9fold purification, at pH 7.5 and 52°C | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | - |
Arthrobacter sp. | D-tagatose | 30% yield after 36 h at 50°C | ? | |
D-galactose | - |
Arthrobacter sp. 22c | D-tagatose | 30% yield after 36 h at 50°C | ? | |
L-arabinose | - |
Arthrobacter sp. | L-ribulose | - |
? | |
L-arabinose | - |
Arthrobacter sp. 22c | L-ribulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 55000, SDS-PAGE | Arthrobacter sp. |
homohexamer | 6 * 55183, calculated from amino acid sequence | Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
- |
Arthrobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the enzyme exhibits over 60% of maximum activity at 30°C | Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | over 90% of the initial activity is retained after 72 h incubation at 50°C. At 60 and 65°C, over 50% of initial activity is lost in incubations of 60 and 30 min, respectively. The enzyme is completely inactivated by 15 min at 70°C | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | the enzyme maintains over 90% of maximum activity in a pH range of 5.0 to 9.0 | Arthrobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
over 90% of the initial activity is retained after 72 h incubation at pH 7.0 | Arthrobacter sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arthrobacter sp. | calculated from amino acid sequence | - |
5.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
D-galactose | at pH 7.0 and 50°C | Arthrobacter sp. |