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Literature summary for 5.3.1.24 extracted from

  • Hoch, S.O.
    Isolation and characterization of two tryptophan biosynthetic enzymes, indoleglycerol phosphate synthase and phosphoribosyl anthranilate isomerase, from Bacillus subtilis (1979), J. Bacteriol., 139, 362-368.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.24

General Stability

General Stability Organism
10-15% v/v glycerol stabilizes Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21800
-
 1 * 21800, SDS-PAGE Bacillus subtilis
26300
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate Bacillus subtilis involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate Bacillus subtilis T3 involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis
-
 not associated with indole-3-glycerol-phosphate synthetase
-
Bacillus subtilis T3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.15
-
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
4°C, 0.8 M sucrose, 7 days, 20% loss of activity, 15 days, 65% loss of activity Bacillus subtilis
4°C, 10-15% v/v glycerol, 6-15 days, average of 24% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Bacillus subtilis T3 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Bacillus subtilis T3 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 21800, SDS-PAGE Bacillus subtilis