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Literature summary for 5.3.1.20 extracted from

  • Elbein, A.D.; Izumori, K.
    D-Ribose isomerase (1982), Methods Enzymol., 89, 547-550.
    View publication on PubMed
Search for more literature for 5.3.1.20

General Stability

General Stability Organism
sensitive to freezing and thawing Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 D-ribose
-
 Mycolicibacterium smegmatis
5.3
-
 L-Lyxose
-
 Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, Km: 0.0004 mM Mycolicibacterium smegmatis
Mg2+ divalent cation required, Km: 0.018 mM Mycolicibacterium smegmatis
Mn2+ divalent cation required for activity, Mn2+ is the best activator, Km: 0.0001 mM Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycolicibacterium smegmatis

Storage Stability

Storage Stability Organism
5°C, crystalline enzyme in 44% ammonium sulphate, stable for at least 1 month Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Allose
-
 Mycolicibacterium smegmatis ?
-
 ?
D-Lyxose
-
 Mycolicibacterium smegmatis ?
-
 ?
D-ribose
-
 Mycolicibacterium smegmatis D-ribulose
-
 ?
L-lyxose
-
 Mycolicibacterium smegmatis ?
-
 ?
L-Rhamnose
-
 Mycolicibacterium smegmatis L-Rhamnulose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 42000-44000, SDS-PAGE in presence of Mn2+ Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Mycolicibacterium smegmatis