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Literature summary for 5.3.1.14 extracted from

  • Bai, W.; Shen, J.; Zhu, Y.; Men, Y.; Sun, Y.; Ma, Y.
    Characteristics and kinetic properties of L-rhamnose isomerase from Bacillus subtilis by isothermal titration calorimetry for the production of D-allose (2015), Food Sci. Technol. Res., 21, 13-22 .
No PubMed abstract available
Search for more literature for 5.3.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM, 68% inhibition Bacillus subtilis
Ca2+ 1 mM, 64% inhibition Bacillus subtilis
Cu2+ 1 mM, 45% inhibition Bacillus subtilis
K+ 1 mM, 68% inhibition Bacillus subtilis
Na+ 1 mM, 68% inhibition Bacillus subtilis
Na2B4O7 1 mM, 90% inhibition Bacillus subtilis
NH4+ 1 mM, 67% inhibition Bacillus subtilis
Zn2+ 1 mM, 64% inhibition Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
 L-rhamnose pH 8.5, 60°C Bacillus subtilis
5.98
-
 D-allose pH 8.5, 60°C Bacillus subtilis
8
-
 L-Mannose pH 8.5, 60°C Bacillus subtilis
8.64
-
 D-ribose pH 8.5, 60°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 10fold activiation Bacillus subtilis
Mg2+ 1 mM, 2.9fold activiation Bacillus subtilis
Mn2+ 1 mM, 47fold activation Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O05264
-
-
Bacillus subtilis 168 O05264
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose
-
 Bacillus subtilis D-psicose
-
 r
D-allose
-
 Bacillus subtilis 168 D-psicose
-
 r
D-psicose
-
 Bacillus subtilis D-allose
-
 r
D-psicose
-
 Bacillus subtilis 168 D-allose
-
 r
D-ribose
-
 Bacillus subtilis D-ribulose
-
 r
D-ribose
-
 Bacillus subtilis 168 D-ribulose
-
 r
L-Mannose
-
 Bacillus subtilis L-Fructose
-
 r
L-Mannose
-
 Bacillus subtilis 168 L-Fructose
-
 r
L-Rhamnose
-
 Bacillus subtilis L-Rhamnulose
-
 r
L-Rhamnose
-
 Bacillus subtilis 168 L-Rhamnulose
-
 r

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 4 h, 85% residual activity Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.74
-
 D-allose pH 8.5, 60°C Bacillus subtilis
7.7
-
 L-rhamnose pH 8.5, 60°C Bacillus subtilis
10.5
-
 L-Mannose pH 8.5, 60°C Bacillus subtilis
30.7
-
 D-ribose pH 8.5, 60°C Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.12
-
 D-allose pH 8.5, 60°C Bacillus subtilis
1.3
-
 L-Mannose pH 8.5, 60°C Bacillus subtilis
3.6
-
 D-ribose pH 8.5, 60°C Bacillus subtilis
15.75
-
 L-rhamnose pH 8.5, 60°C Bacillus subtilis