Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Dictyoglomus turgidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.6 | - |
L-rhamnose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
33.4 | - |
L-Lyxose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
36.8 | - |
L-Mannose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
54.5 | - |
D-ribose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
61.5 | - |
D-allose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
63 | - |
L-xylulose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
73.2 | - |
L-Fructose | at pH 8.0 and 75°C | Dictyoglomus turgidum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Dictyoglomus turgidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46695 | - |
4 * 46695, calculated from amino acid sequence | Dictyoglomus turgidum |
47000 | - |
4 * 47000, His6-tagged enzyme, SDS-PAGE | Dictyoglomus turgidum |
185000 | - |
gel filtration | Dictyoglomus turgidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Rhamnose | Dictyoglomus turgidum | best substrate, conversion yield of 43% | L-Rhamnulose | - |
? | |
L-Rhamnose | Dictyoglomus turgidum DSM 6724 | best substrate, conversion yield of 43% | L-Rhamnulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyoglomus turgidum | B8E1U6 | - |
- |
Dictyoglomus turgidum DSM 6724 | B8E1U6 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap HP column chromatography, and gel filtration | Dictyoglomus turgidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Dictyoglomus turgidum | D-psicose | - |
? | |
D-allose | - |
Dictyoglomus turgidum DSM 6724 | D-psicose | - |
? | |
D-ribose | worst substrate | Dictyoglomus turgidum | D-ribulose | - |
? | |
D-ribose | worst substrate | Dictyoglomus turgidum DSM 6724 | D-ribulose | - |
? | |
L-fructose | - |
Dictyoglomus turgidum | L-mannose | - |
? | |
L-lyxose | second best substrate | Dictyoglomus turgidum | L-xylulose | - |
? | |
L-lyxose | second best substrate | Dictyoglomus turgidum DSM 6724 | L-xylulose | - |
? | |
L-Mannose | - |
Dictyoglomus turgidum | L-Fructose | - |
? | |
L-Mannose | - |
Dictyoglomus turgidum DSM 6724 | L-Fructose | - |
? | |
L-Rhamnose | best substrate, conversion yield of 43% | Dictyoglomus turgidum | L-Rhamnulose | - |
? | |
L-Rhamnose | best substrate, conversion yield of 43% | Dictyoglomus turgidum DSM 6724 | L-Rhamnulose | - |
? | |
L-xylulose | - |
Dictyoglomus turgidum | L-lyxose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 46695, calculated from amino acid sequence | Dictyoglomus turgidum |
homotetramer | 4 * 47000, His6-tagged enzyme, SDS-PAGE | Dictyoglomus turgidum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
maximum activities of both the free and immobilized enzymes for L-rhamnose isomerization | Dictyoglomus turgidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
the half-lives of the free and immobilized enzymes are 28 and 112 h at pH 8.0 and 75°C in the presence of 1 mM Mn2+ | Dictyoglomus turgidum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
49 | - |
D-ribose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
81 | - |
D-allose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
116 | - |
L-Fructose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
130 | - |
L-xylulose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
155 | - |
L-Mannose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
195 | - |
L-rhamnose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
200 | - |
L-Lyxose | at pH 8.0 and 75°C | Dictyoglomus turgidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
maximum activities of both the free and immobilized enzymes for L-rhamnose isomerization | Dictyoglomus turgidum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
the half-lives of the free and immobilized enzymes are 28 and 112 h at pH 8.0 and 75°C in the presence of 1 mM Mn2+ | Dictyoglomus turgidum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
D-ribose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
1.3 | - |
D-allose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
1.6 | - |
L-Fructose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
2.1 | - |
L-xylulose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
4.2 | - |
L-Mannose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
6 | - |
L-Lyxose | at pH 8.0 and 75°C | Dictyoglomus turgidum | |
7.9 | - |
L-rhamnose | at pH 8.0 and 75°C | Dictyoglomus turgidum |