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Literature summary for 5.3.1.14 extracted from

  • Kim, Y.S.; Shin, K.C.; Lim, Y.R.; Oh, D.K.
    Characterization of a recombinant L-rhamnose isomerase from Dictyoglomus turgidum and its application for L-rhamnulose production (2013), Biotechnol. Lett., 35, 259-264.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Dictyoglomus turgidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
24.6
-
L-rhamnose at pH 8.0 and 75°C Dictyoglomus turgidum
33.4
-
L-Lyxose at pH 8.0 and 75°C Dictyoglomus turgidum
36.8
-
L-Mannose at pH 8.0 and 75°C Dictyoglomus turgidum
54.5
-
D-ribose at pH 8.0 and 75°C Dictyoglomus turgidum
61.5
-
D-allose at pH 8.0 and 75°C Dictyoglomus turgidum
63
-
L-xylulose at pH 8.0 and 75°C Dictyoglomus turgidum
73.2
-
L-Fructose at pH 8.0 and 75°C Dictyoglomus turgidum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Dictyoglomus turgidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46695
-
4 * 46695, calculated from amino acid sequence Dictyoglomus turgidum
47000
-
4 * 47000, His6-tagged enzyme, SDS-PAGE Dictyoglomus turgidum
185000
-
gel filtration Dictyoglomus turgidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Rhamnose Dictyoglomus turgidum best substrate, conversion yield of 43% L-Rhamnulose
-
?
L-Rhamnose Dictyoglomus turgidum DSM 6724 best substrate, conversion yield of 43% L-Rhamnulose
-
?

Organism

Organism UniProt Comment Textmining
Dictyoglomus turgidum B8E1U6
-
-
Dictyoglomus turgidum DSM 6724 B8E1U6
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap HP column chromatography, and gel filtration Dictyoglomus turgidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose
-
Dictyoglomus turgidum D-psicose
-
?
D-allose
-
Dictyoglomus turgidum DSM 6724 D-psicose
-
?
D-ribose worst substrate Dictyoglomus turgidum D-ribulose
-
?
D-ribose worst substrate Dictyoglomus turgidum DSM 6724 D-ribulose
-
?
L-fructose
-
Dictyoglomus turgidum L-mannose
-
?
L-lyxose second best substrate Dictyoglomus turgidum L-xylulose
-
?
L-lyxose second best substrate Dictyoglomus turgidum DSM 6724 L-xylulose
-
?
L-Mannose
-
Dictyoglomus turgidum L-Fructose
-
?
L-Mannose
-
Dictyoglomus turgidum DSM 6724 L-Fructose
-
?
L-Rhamnose best substrate, conversion yield of 43% Dictyoglomus turgidum L-Rhamnulose
-
?
L-Rhamnose best substrate, conversion yield of 43% Dictyoglomus turgidum DSM 6724 L-Rhamnulose
-
?
L-xylulose
-
Dictyoglomus turgidum L-lyxose
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 46695, calculated from amino acid sequence Dictyoglomus turgidum
homotetramer 4 * 47000, His6-tagged enzyme, SDS-PAGE Dictyoglomus turgidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
maximum activities of both the free and immobilized enzymes for L-rhamnose isomerization Dictyoglomus turgidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
the half-lives of the free and immobilized enzymes are 28 and 112 h at pH 8.0 and 75°C in the presence of 1 mM Mn2+ Dictyoglomus turgidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
49
-
D-ribose at pH 8.0 and 75°C Dictyoglomus turgidum
81
-
D-allose at pH 8.0 and 75°C Dictyoglomus turgidum
116
-
L-Fructose at pH 8.0 and 75°C Dictyoglomus turgidum
130
-
L-xylulose at pH 8.0 and 75°C Dictyoglomus turgidum
155
-
L-Mannose at pH 8.0 and 75°C Dictyoglomus turgidum
195
-
L-rhamnose at pH 8.0 and 75°C Dictyoglomus turgidum
200
-
L-Lyxose at pH 8.0 and 75°C Dictyoglomus turgidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
maximum activities of both the free and immobilized enzymes for L-rhamnose isomerization Dictyoglomus turgidum

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
the half-lives of the free and immobilized enzymes are 28 and 112 h at pH 8.0 and 75°C in the presence of 1 mM Mn2+ Dictyoglomus turgidum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
D-ribose at pH 8.0 and 75°C Dictyoglomus turgidum
1.3
-
D-allose at pH 8.0 and 75°C Dictyoglomus turgidum
1.6
-
L-Fructose at pH 8.0 and 75°C Dictyoglomus turgidum
2.1
-
L-xylulose at pH 8.0 and 75°C Dictyoglomus turgidum
4.2
-
L-Mannose at pH 8.0 and 75°C Dictyoglomus turgidum
6
-
L-Lyxose at pH 8.0 and 75°C Dictyoglomus turgidum
7.9
-
L-rhamnose at pH 8.0 and 75°C Dictyoglomus turgidum