Application | Comment | Organism |
---|---|---|
food industry | L-rhamnose isomerase is used in rare sugar production | Thermoanaerobacterium saccharolyticum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Thermoanaerobacterium saccharolyticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.53 | - |
D-ribose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
3.53 | - |
L-rhamnose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
45.2 | - |
L-Lyxose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
58.9 | - |
L-Mannose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
121 | - |
D-allose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme shows 1% activity in the presence of 1 mM Ca2+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
Co2+ | 100% activity in the presence of 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
Mg2+ | the enzyme shows 9% activity in the presence of 1 mM Mg2+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
Mn2+ | the enzyme shows 61% activity in the presence of 1 mM Mn2+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
additional information | the enzyme shows null activity in the absence of any ions, as well as in the presence of Cu2+ and K+ | Thermoanaerobacterium saccharolyticum | |
Na+ | the enzyme shows 19% activity in the presence of 1 mM Na+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
Zn2+ | the enzyme shows 2% activity in the presence of 1 mM Zn2+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
48960 | - |
x * 48960, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium saccharolyticum | D5LNE5 | - |
- |
Thermoanaerobacterium saccharolyticum NTOU1 | D5LNE5 | - |
- |
Purification (Comment) | Organism |
---|---|
heat treatment, nucleic acid precipitation, and Q-Sepharose column chromatography | Thermoanaerobacterium saccharolyticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65.6 | - |
crude extract, at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |
197 | - |
after 3fold purification, at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Thermoanaerobacterium saccharolyticum | D-psicose | - |
r | |
D-allose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | D-psicose | - |
r | |
D-ribose | - |
Thermoanaerobacterium saccharolyticum | D-ribulose | - |
? | |
D-ribose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | D-ribulose | - |
? | |
L-lyxose | - |
Thermoanaerobacterium saccharolyticum | L-xylulose | - |
r | |
L-lyxose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | L-xylulose | - |
r | |
L-Mannose | - |
Thermoanaerobacterium saccharolyticum | L-Fructose | - |
r | |
L-Mannose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | L-Fructose | - |
r | |
L-Rhamnose | - |
Thermoanaerobacterium saccharolyticum | L-Rhamnulose | - |
r | |
L-Rhamnose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | L-Rhamnulose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
? | x * 48960, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
Synonyms | Comment | Organism |
---|---|---|
L-RhI | - |
Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermoanaerobacterium saccharolyticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | the activity is fully retained after 2 h incubation at 40-70°C | Thermoanaerobacterium saccharolyticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.5 | - |
D-ribose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
30 | - |
L-Mannose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
33.9 | - |
D-allose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
180 | - |
L-rhamnose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
240 | - |
L-Lyxose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermoanaerobacterium saccharolyticum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | the enzyme is stable at pH values ranging from 5.0 to 9.0 | Thermoanaerobacterium saccharolyticum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
D-ribose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
0.28 | - |
D-allose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
0.51 | - |
L-Mannose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3 | - |
L-Lyxose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
50.8 | - |
L-rhamnose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |