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Literature summary for 5.3.1.14 extracted from

  • Park, C.S.; Yeom, S.J.; Lim, Y.R.; Kim, Y.S.; Oh, D.K.
    Characterization of a recombinant thermostable L-rhamnose isomerase from Thermotoga maritima ATCC 43589 and its application in the production of L-lyxose and L-mannose (2010), Biotechnol. Lett., 32, 1947-1953.
    View publication on PubMed
Search for more literature for 5.3.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
37
-
 L-rhamnose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
69
-
 L-Lyxose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
76
-
 L-Mannose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
111
-
 L-xylulose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
123
-
 L-Fructose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ enzyme activity is the highest with Mn2+, the optimal Mn2+ concentration is 1 mM (6.7fold increase activity relative to no treatment) Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45527
-
 4 * 45527, calculated from amino acid sequence Thermotoga maritima
46000
-
 4 * 46000, SDS-PAGE Thermotoga maritima
184000
-
 gel filtration Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima ATCC 43589
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
1.1
-
 using D-psicose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
2.8
-
 using D-ribose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.1
-
 using L-fructose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.3
-
 using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
6.7
-
 using D-allose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
6.8
-
 using D-ribulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
8.6
-
 using L-xylulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
15
-
 using L-mannose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
20
-
 using L-lyxose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
55
-
 using L-rhamnose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose
-
 Thermotoga maritima D-psicose
-
 r
D-gulose
-
 Thermotoga maritima D-sorbose
-
 r
D-ribose
-
 Thermotoga maritima D-ribulose
-
 r
D-ribose
-
 Thermotoga maritima ATCC 43589 D-ribulose
-
 r
L-fructose
-
 Thermotoga maritima L-mannose
-
 r
L-lyxose
-
 Thermotoga maritima L-xylulose
-
 r
L-lyxose
-
 Thermotoga maritima ATCC 43589 L-xylulose
-
 r
L-Mannose
-
 Thermotoga maritima L-Fructose
-
 r
L-Mannose
-
 Thermotoga maritima ATCC 43589 L-Fructose
-
 r
L-Rhamnose highest specific activity Thermotoga maritima L-Rhamnulose
-
 r
L-Rhamnose highest specific activity Thermotoga maritima ATCC 43589 L-Rhamnulose
-
 r
L-xylulose
-
 Thermotoga maritima L-lyxose
-
 r
additional information no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate Thermotoga maritima ?
-
 ?
additional information no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate Thermotoga maritima ATCC 43589 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 46000, SDS-PAGE Thermotoga maritima
tetramer 4 * 45527, calculated from amino acid sequence Thermotoga maritima

Synonyms

Synonyms Comment Organism
RhaA
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75 95 the half-lives of the enzyme at 75°C, 80°C, 85°C, 90°C and 95°C are 773 h, 347 h, 187 h, 118 h, and 65 h, respectively Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
146
-
 L-rhamnose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
274
-
 L-Fructose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
281
-
 L-xylulose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
442
-
 L-Mannose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
462
-
 L-Lyxose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
8
-
-
 Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.23
-
 L-Fructose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
2.55
-
 L-xylulose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
5.82
-
 L-Mannose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
6.72
-
 L-Lyxose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima
39.36
-
 L-rhamnose purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) Thermotoga maritima