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Literature summary for 5.3.1.14 extracted from

  • Takata, G.; Uechi, K.; Taniguchi, E.; Kanbara, Y.; Yoshihara, A.; Morimoto, K.; Izumori, K.
    Characterization of Mesorhizobium loti L-rhamnose isomerase and its application to L-talose production (2011), Biosci. Biotechnol. Biochem., 75, 1006-1009.
    View publication on PubMed
Search for more literature for 5.3.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Mesorhizobium loti

Inhibitors

Inhibitors Comment Organism Structure
EDTA enzyme activity is lost after EDTA treatment Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3.4 L-Mannose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5
-
 L-rhamnose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
5.25
-
 L-talose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
6.18
-
 D-ribose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
7.11
-
 D-allose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
23.5
-
 L-Lyxose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ highest activity in the presence of Mn2+ Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46946
-
 x * 46946, calculated from amino acid sequence Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti
-
-
-
Mesorhizobium loti Tono
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Allose
-
 Mesorhizobium loti ?
-
 ?
D-Allose
-
 Mesorhizobium loti Tono ?
-
 ?
D-allose
-
 Mesorhizobium loti D-talose
-
 ?
D-Arabinose slight activity Mesorhizobium loti ?
-
 ?
D-Galactose slight activity Mesorhizobium loti ?
-
 ?
D-glucose slight activity Mesorhizobium loti ?
-
 ?
D-Lyxose slight activity Mesorhizobium loti ?
-
 ?
D-Mannose
-
 Mesorhizobium loti ?
-
 ?
D-Mannose slight activity Mesorhizobium loti ?
-
 ?
D-ribose
-
 Mesorhizobium loti D-ribulose
-
 ?
D-ribose
-
 Mesorhizobium loti Tono D-ribulose
-
 ?
D-Ribose
-
 Mesorhizobium loti ?
-
 ?
D-talose slight activity Mesorhizobium loti D-allose
-
 ?
D-talose slight activity Mesorhizobium loti ?
-
 ?
L-Arabinose slight activity Mesorhizobium loti ?
-
 ?
L-galactose slight activity Mesorhizobium loti ?
-
 ?
L-lyxose
-
 Mesorhizobium loti L-xylulose
-
 r
L-lyxose
-
 Mesorhizobium loti Tono L-xylulose
-
 r
L-lyxose
-
 Mesorhizobium loti ?
-
 r
L-Mannose
-
 Mesorhizobium loti L-Fructose
-
 ?
L-Mannose
-
 Mesorhizobium loti Tono L-Fructose
-
 ?
L-mannose
-
 Mesorhizobium loti ?
-
 ?
L-Rhamnose highest activity Mesorhizobium loti L-Rhamnulose
-
 ?
L-Rhamnose highest activity Mesorhizobium loti Tono L-Rhamnulose
-
 ?
L-ribose slight activity Mesorhizobium loti ?
-
 ?
L-talose
-
 Mesorhizobium loti ?
-
 ?
L-xylulose
-
 Mesorhizobium loti L-lyxose
-
 r

Subunits

Subunits Comment Organism
? x * 46946, calculated from amino acid sequence Mesorhizobium loti

Synonyms

Synonyms Comment Organism
L-RhI
-
 Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Mesorhizobium loti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 after incubation at 50°C in glycine-NaOH buffer (pH 9.0) for 1 h, approximately 70% of enzyme activity is retained Mesorhizobium loti
50
-
 after incubation at 50°C in glycine–NaOH buffer (pH 9.0) for 1 h, approximately 70% of enzyme activity is retained Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 D-ribose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
1.3
-
 D-allose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
7
-
 L-talose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
10
-
 L-Mannose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
50.5
-
 L-rhamnose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
73.8
-
 L-Lyxose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Mesorhizobium loti

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 the enzyme remains stable after 24 h over a broad pH range from pH 5.0-11.0 Mesorhizobium loti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
 D-allose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
0.4
-
 L-Mannose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
0.8
-
 D-ribose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
1.3
-
 L-talose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
3.1
-
 L-Lyxose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti
10.1
-
 L-rhamnose in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C Mesorhizobium loti