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Literature summary for 5.3.1.14 extracted from

  • Bhuiyan, S.H.; Itami, Y.; Izumori, K.
    Immobilization of L-rhamnose isomerase and its application in L-mannose production from L-fructose (1997), J. Ferment. Bioeng., 84, 558-562.
No PubMed abstract available
Search for more literature for 5.3.1.14

Application

Application Comment Organism
synthesis large scale production of L-mannose from L-fructose by immobilized enzyme Pseudomonas sp.

General Stability

General Stability Organism
immobilized enzyme retains about 90% of the initial activity even after five repeated uses in a batch reaction Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ immobilized as well as free enzyme requires Mn2+ for maximal activity Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. LL172
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Mannose only reverse direction described. At equilibrium the production of L-mannose from L-fructose is 30% Pseudomonas sp. L-Fructose
-
 ?
L-Mannose only reverse direction described. At equilibrium the production of L-mannose from L-fructose is 30% Pseudomonas sp. LL172 L-Fructose
-
 ?
L-Rhamnose
-
 Pseudomonas sp. L-Rhamnulose
-
 ?
L-Rhamnose
-
 Pseudomonas sp. LL172 L-Rhamnulose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 immobilized enzyme Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min stable, glycine-NaOH buffer, immobilized enzyme Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 11 immobilized enzyme Pseudomonas sp.
9
-
 free enzyme Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 11 pH-range of optimal activity, immobilized enzyme Pseudomonas sp.
7 11 pH 7: about 50% of maximal activity, pH 11: about 40% of maximal activity, free enzyme Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 4°C, 24 h, immobilized enzyme remains stable Pseudomonas sp.