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Literature summary for 5.3.1.14 extracted from
- Ueber den Abbau der Desoxyzucker durch Bacterienenzyme. I. L-Rhamnose-isomerase aus Lactobacillus plantarum (1963), Biochem. Z., 339, 145-153.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl reagents | activate | Lactiplantibacillus plantarum |  |
| thioglycolate | best activator among sulfhydryl reagents | Lactiplantibacillus plantarum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Lactiplantibacillus plantarum | |
| Cu2+ | - |
Lactiplantibacillus plantarum | |
| Ni2+ | - |
Lactiplantibacillus plantarum | |
| Zn2+ | - |
Lactiplantibacillus plantarum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | weak activation | Lactiplantibacillus plantarum | |
| Mn2+ | activates | Lactiplantibacillus plantarum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactiplantibacillus plantarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31.8 | - |
- |
Lactiplantibacillus plantarum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, stable | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-Deoxy-L-rhamnose | 0.6% of the activity with L-rhamnose | Lactiplantibacillus plantarum | ? | - |
? | |
| L-Mannose | 0.6% of the activity with L-rhamnose | Lactiplantibacillus plantarum | L-Fructose | - |
? | |
| L-Rhamnose | - |
Lactiplantibacillus plantarum | L-Rhamnulose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
50 h stable, partially purified enzyme | Lactiplantibacillus plantarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Lactiplantibacillus plantarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6: about 65% of maximal activity, pH 9.5: about 80% of maximal activity, pH 10.0: about 15% of maximal activity | Lactiplantibacillus plantarum |
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Release 2023.1 (January 2023)
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