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Literature summary for 5.3.1.13 extracted from

  • Cech, D.; Wang, P.F.; Holler, T.P.; Woodard, R.W.
    Analysis of the arabinose-5-phosphate isomerase of Bacteroides fragilis provides insight into regulation of single-domain arabinose phosphate isomerases (2014), J. Bacteriol., 196, 2861-2868 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.13

Inhibitors

Inhibitors Comment Organism Structure
cytidine 5'-monophospho-3-deoxy-D-manno-2-octulosonic acid final product of the 3-deoxy-D-manno-2-octulosonic acid biosynthesis pathway Bacteroides fragilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.485
-
 D-ribulose 5-phosphate pH 8.5, 37°C Bacteroides fragilis
34.5
-
 D-arabinose 5-phosphate pH 8.5, 37°C Bacteroides fragilis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information purified protein does not contain metal ions Bacteroides fragilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85900
-
 gel fitration Bacteroides fragilis

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis Q5LIW1
-
-
Bacteroides fragilis DSM 2151 Q5LIW1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Arabinose 5-phosphate
-
 Bacteroides fragilis D-Ribulose 5-phosphate
-
 r
D-Arabinose 5-phosphate
-
 Bacteroides fragilis DSM 2151 D-Ribulose 5-phosphate
-
 r
D-ribulose 5-phosphate
-
 Bacteroides fragilis D-arabinose 5-phosphate
-
 r
D-ribulose 5-phosphate
-
 Bacteroides fragilis DSM 2151 D-arabinose 5-phosphate
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 21000, SDS-PAGE, 4 * 21810, mass spectrometry Bacteroides fragilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.5
-
 D-ribulose 5-phosphate pH 8.5, 37°C Bacteroides fragilis
253
-
 D-arabinose 5-phosphate pH 8.5, 37°C Bacteroides fragilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 9 broad Bacteroides fragilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0019
-
 cytidine 5'-monophospho-3-deoxy-D-manno-2-octulosonic acid pH 8.5, 37°C Bacteroides fragilis

General Information

General Information Comment Organism
physiological function expression in Escherichia coli complements an arabinose-5-phosphate isomerase deficient strain Bacteroides fragilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.3
-
 D-arabinose 5-phosphate pH 8.5, 37°C Bacteroides fragilis
38
-
 D-ribulose 5-phosphate pH 8.5, 37°C Bacteroides fragilis