Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
H190A | inactive | Pseudomonas aeruginosa |
H85A | inactive | Pseudomonas aeruginosa |
K56A | the mutant shows 0.31% residual activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Pseudomonas aeruginosa |
34067 | - |
x * 34067, calculated from amino acid sequence | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Arabinose 5-phosphate | Pseudomonas aeruginosa | - |
D-Ribulose 5-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.092 | - |
mutant enzyme K56A, in Tris·HCl, (pH 8.5, 50 mM), at 37°C | Pseudomonas aeruginosa |
29.5 | - |
recombinant wild type enzyme, in Tris·HCl, (pH 8.5, 50 mM), at 37°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-threo-pentofuranose 5-phosphate | the catalytic efficiency is about 1000times lower than for the natural substrate D-arabinose 5-phosphate | Pseudomonas aeruginosa | 3-deoxy-D-glycero-pentulose 5-phosphate | - |
? | |
4-deoxy-D-threo-pentofuranose 5-phosphate | the catalytic efficiency is comparable with the natural substrate D-arabinose 5-phosphate | Pseudomonas aeruginosa | 4-deoxy-D-glycero-pentulose 5-phosphate | - |
? | |
D-Arabinose 5-phosphate | - |
Pseudomonas aeruginosa | D-Ribulose 5-phosphate | - |
r | |
additional information | no activity with D-ribose 5-phosphate | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Pseudomonas aeruginosa |
? | x * 34067, calculated from amino acid sequence | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
API | - |
Pseudomonas aeruginosa |
D-arabinose-5P isomerase | - |
Pseudomonas aeruginosa |
KdsD | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of 3-deoxy-D-manno-oct-2-ulosonic acid and thus of lipopolysaccharide | Pseudomonas aeruginosa |