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Literature summary for 5.3.1.13 extracted from

  • Gourlay, L.J.; Sommaruga, S.; Nardini, M.; Sperandeo, P.; Deho, G.; Polissi, A.; Bolognesi, M.
    Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography (2010), Protein Sci., 19, 2430-2439.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BB-8 cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme K59A, sitting drop vapor diffusion method, using 20% PEG 8K and 0.1 M HEPES, pH 7.5 Escherichia coli

Protein Variants

Protein Variants Comment Organism
H88A the enzyme site-specific mutant is able to catalyze the isomerization of A5P to R5P in vitro with 9.5% of the wild type protein activity Escherichia coli
K59A catalytically inactive Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 full-length protein, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P45395
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelate affinity column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29.55
-
 pH and temperature not specified in the publication Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Arabinose 5-phosphate
-
 Escherichia coli D-Ribulose 5-phosphate
-
 r

Subunits

Subunits Comment Organism
tetramer x-ray crystallography Escherichia coli

Synonyms

Synonyms Comment Organism
API
-
 Escherichia coli
D-arabinose-5-phosphate isomerase
-
 Escherichia coli
KdsD
-
 Escherichia coli