BRENDA - Enzyme Database
show all sequences of 5.3.1.13

Inhibition of arabinose 5-phosphate isomerase. An approach to the inhibition of bacterial lipopolysaccharide biosynthesis

Bigham, E.C.; Gragg, Ch.E.; Hall, W.R.; Kelsey, J.E.; Mallory, W.R.; Richardson, C.B.; Benedict, C.; Ray, P.H.; J. Med. Chem. 27, 717-726 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Erythronic acid 4-phosphate
best inhibitor
Escherichia coli
Phosphorylated sugars
in a series of nonphosphorylated sugars, the order of decreasing inhibitory activity is as follows: aldonic acids, alditols, aldoses
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
0.15
ribulose 5-phosphate
-
Escherichia coli
0.1
0.3
D-arabinose 5-phosphate
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
135000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Escherichia coli
key enzyme in synthesis of lipopolysaccharides
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Reaction
Reaction
Commentary
Organism
D-Arabinose 5-phosphate = D-ribulose 5-phosphate
mechanism is envisioned to involve an enediol intermediate
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Arabinose 5-phosphate
r
2625
Escherichia coli
D-Ribulose 5-phosphate
-
2625
Escherichia coli
-
additional information
key enzyme in synthesis of lipopolysaccharides
2625
Escherichia coli
?
-
-
-
-
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Erythronic acid 4-phosphate
best inhibitor
Escherichia coli
Phosphorylated sugars
in a series of nonphosphorylated sugars, the order of decreasing inhibitory activity is as follows: aldonic acids, alditols, aldoses
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
0.15
ribulose 5-phosphate
-
Escherichia coli
0.1
0.3
D-arabinose 5-phosphate
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
135000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Escherichia coli
key enzyme in synthesis of lipopolysaccharides
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Arabinose 5-phosphate
r
2625
Escherichia coli
D-Ribulose 5-phosphate
-
2625
Escherichia coli
-
additional information
key enzyme in synthesis of lipopolysaccharides
2625
Escherichia coli
?
-
-
-
-
Other publictions for EC 5.3.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748109
Cech
Identification of a D-arabino ...
Clostridium tetani
J. Bacteriol.
199
e00246
2017
-
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1
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2
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1
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6
-
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3
1
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2
1
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1
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2
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1
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3
1
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2
1
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1
1
-
2
2
749376
Qu
Expression, purification and ...
Arabidopsis thaliana
Sheng Wu Gong Cheng Xue Bao
32
1060-1069
2016
1
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1
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2
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1
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1
1
1
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1
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-
746617
Chiu
Structural analysis of arabin ...
Bacteroides fragilis, Bacteroides fragilis DSM 2151
Acta Crystallogr. Sect. D
70
2640-2651
2014
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1
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4
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747252
Gabrielli
Arabinose 5-phosphate isomera ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644
Bioorg. Med. Chem.
22
2576-2583
2014
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3
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2
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748087
Cech
Analysis of the arabinose-5-p ...
Bacteroides fragilis, Bacteroides fragilis DSM 2151
J. Bacteriol.
196
2861-2868
2014
-
-
-
-
-
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1
2
-
1
1
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7
-
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4
1
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2
1
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1
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1
1
2
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1
1
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-
-
4
1
-
-
-
2
1
-
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1
1
-
2
2
714430
Yep
Enediol mimics as inhibitors o ...
Francisella tularensis
Bioorg. Med. Chem. Lett.
21
2679-2682
2011
-
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1
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8
1
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1
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4
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1
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1
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1
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4
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4
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1
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4
8
4
1
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1
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715380
Mosberg
A unique arabinose 5-phosphate ...
Escherichia coli, Escherichia coli CFT073
J. Bacteriol.
193
2981-2988
2011
1
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1
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3
2
-
9
3
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9
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1
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6
1
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2
1
1
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1
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1
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3
-
2
-
9
3
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1
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6
1
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2
1
1
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2
2
-
2
2
727304
Airoldi
Targeting bacterial membranes: ...
Pseudomonas aeruginosa
ChemBioChem
12
719-727
2011
-
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1
-
3
-
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1
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2
1
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2
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1
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2
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4
1
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1
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3
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1
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1
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4
1
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1
1
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714743
Airoldi
Targeting bacterial membranes: ...
Escherichia coli
Chemistry
16
1897-1902
2010
-
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1
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1
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1
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2
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4
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1
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1
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4
-
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1
1
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-
716854
Gourlay
Probing the active site of the ...
Escherichia coli
Protein Sci.
19
2430-2439
2010
-
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1
1
2
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1
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3
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1
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1
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701812
Yeom
L-ribose production from L-ara ...
Escherichia coli
Appl. Environ. Microbiol.
75
6941-6943
2009
-
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1
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1
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1
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4
1
1
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1
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671856
Meredith
Characterization of Escherichi ...
Escherichia coli, Escherichia coli CFT073
Biochem. J.
395
427-432
2006
1
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1
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4
6
1
5
3
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4
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1
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1
1
6
1
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3
1
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1
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1
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4
-
6
1
5
3
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1
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1
1
6
1
-
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3
1
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676073
Tan
Yersinia pestis YrbH is a mult ...
Yersinia pestis
Mol. Microbiol.
61
861-870
2006
-
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1
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5
-
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2
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2
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660993
D'Aniello
Cephalopod vision involves dic ...
Escherichia coli, Escherichia coli CFT073
Biochem. J.
386
331-340
2005
-
-
1
-
-
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3
2
-
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1
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4
-
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1
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2
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2
1
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1
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3
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2
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2
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1
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662042
Meredith
Identification of GutQ from Es ...
Escherichia coli
J. Bacteriol.
187
6936-6942
2005
1
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1
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1
2
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1
2
1
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2
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1
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1
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2
1
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1
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1
2
1
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1
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2
1
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2
1
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652445
Meredith
Escherichia coli YrbH is a D-a ...
Escherichia coli
J. Biol. Chem.
278
32771-32777
2003
-
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1
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6
2
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2
1
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4
-
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1
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1
1
3
1
-
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2
1
1
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1
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1
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1
6
-
2
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2
1
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1
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1
1
3
1
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2
1
1
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645237
Tzeng
KpsF is the arabinose-5-phosph ...
Neisseria meningitidis
J. Biol. Chem.
277
24103-24113
2002
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1
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1
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1
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4
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1
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2625
Bigham
Inhibition of arabinose 5-phos ...
Escherichia coli
J. Med. Chem.
27
717-726
1984
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2
2
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1
1
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2
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1
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2624
Noltmann
-
Aldose-ketose isomerases ...
Acidipropionibacterium acidipropionici, Escherichia coli
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
6
271-354
1972
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6
3
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3
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3
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3
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6
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2
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2623
Volk
-
D-Arabinose 5-phosphate isomer ...
Acidipropionibacterium acidipropionici, Acidipropionibacterium acidipropionici E214
Methods Enzymol.
9
585-588
1966
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1
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2
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1
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1
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2
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1
1
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1
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1
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1
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2
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1
1
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2622
Volk
-
Purification and properties of ...
Acidipropionibacterium acidipropionici
J. Biol. Chem.
235
1550-1553
1960
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1
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