Literature summary for 5.3.1.12 extracted from

Linster, C.L.; Van Schaftingen, E.
A spectrophotometric assay of D-glucuronate based on Escherichia coli uronate isomerase and mannonate dehydrogenase (2004), Protein Expr. Purif., 37, 352-360.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli BL21(DE3)pLysS	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	0.25 mM, 50% inhibition	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.29	-	D-glucuronate	in absence of added metal or chelator	Escherichia coli
0.31	-	D-glucuronate	in presence of 0.01 mM Zn2+	Escherichia coli
0.41	-	D-glucuronate	in presence of 0.01 mM Co2+	Escherichia coli
1.2	-	D-glucuronate	in presence of 0.1 mM Mn2+	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	stimulation is more potent at lower concentrations than stimulation by Mn2+	Escherichia coli
Mn2+	maximal stimulation is higher than stimulatipn by Zn2+ or Co2+, stimulation at low concentrations is lower than stimulation by Zn2+ and Co2+	Escherichia coli
Zn2+	stimulation is more potent at lower concentrations than stimulation by Mn2+. Vmax is about 3times higher in presence of 0.1 mM Mn2+ than in presence of 0.01 mM Zn2+ or Co2+	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glucuronate	-	Escherichia coli	D-Fructuronate	-	?

Synonyms

Synonyms	Comment	Organism
Uronate isomerase	-	Escherichia coli

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Release 2023.1 (January 2023)