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Literature summary for 5.3.1.1 extracted from

  • Diaz-Mazariegos, S.; Cabrera, N.; Perez-Montfort, R.
    Three unrelated and unexpected amino acids determine the susceptibility of the interface cysteine to a sulfhydryl reagent in the triosephosphate isomerases of two trypanosomes (2018), PLoS ONE, 13, e0189525 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
E26D/T27L/L28F/A30S/L100A/Q115A mutant construcuted to produce a TIM with the inactivation susceptibility pattern of Trypanosoma brucei brucei enzyme, shows a similar resistance pattern to the inactivation with methylmethane thiosulfonate as wild-type Trypanosoma brucei brucei TIM Trypanosoma cruzi
E26D/T27L/L28F/A30S/T32S/L100A/Q115A mutant construcuted to produce a TIM with the inactivation susceptibility pattern of Trypanosoma brucei brucei enzyme, shows a similar resistance pattern to the inactivation with methylmethane thiosulfonate as wild-type Trypanosoma brucei brucei TIM Trypanosoma cruzi
E26D/T27L/L28F/L100A/Q115A mutant construcuted to produce a TIM with the inactivation susceptibility pattern of Trypanosoma brucei brucei enzyme, shows a similar resistance pattern to the inactivation with methylmethane thiosulfonate as wild-type Trypanosoma brucei brucei TIM Trypanosoma cruzi
F28L/A100L/A115Q mutant constructed to mimick the methylmethane sulfonate inactivation pattern of the Trypanosoma cruzi enzyme. Protein is similarly susceptibel to methylmethane sulfonate as the Trypanosoma cruzi enzyme Trypanosoma brucei brucei
L28F/L100A/Q115A mutant construcuted to produce a TIM with the inactivation susceptibility pattern of Trypanosoma brucei brucei enzyme. Protein is more resistant to the inactivation with methylmethane thiosulfonate as wild-type Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
methylmethane thiosulfonate inactivation by sulfhydroxylation Trypanosoma brucei brucei
methylmethane thiosulfonate inactivation by sulfhydroxylation Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei P04789
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Trypanosoma cruzi P52270
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