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Literature summary for 5.3.1.1 extracted from

  • Zhai, X.; Amyes, T.L.; Richard, J.P.
    Role of loop-clamping side chains in catalysis by triosephosphate isomerase (2015), J. Am. Chem. Soc., 137, 15185-15197 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
S211A mutation eliminates intraloop hydrogen bonds to the side-chain hydroxyl, 60fold decrease in kcat/Km Saccharomyces cerevisiae
S211G mutation eliminates intraloop hydrogen bonds to the side-chain hydroxyl, leading to small changes in the kinetic parameters Saccharomyces cerevisiae
Y208A main effect of mutations is to cause a reduction in the total intrinsic dianion binding energy Saccharomyces cerevisiae
Y208F mutation eliminates the intraloop hydrogen bond between the hydroxyl group of Y208 and the amide nitrogen of A176. Enzyme activity is reduced by ca. 50fold compared to the Y208A and Y208S mutants Saccharomyces cerevisiae
Y208S main effect of mutations is to cause a reduction in the total intrinsic dianion binding energy Saccharomyces cerevisiae
Y208T main effect of mutations is to cause a reduction in the total intrinsic dianion binding energy Saccharomyces cerevisiae
Y208T/S211G tenfold decrease in kcat/Km Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
arsenate
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
D-glyceraldehyde 3-phosphate mutant Y208T/S211G, pH 7.5, 25°C Saccharomyces cerevisiae
1
-
D-glyceraldehyde 3-phosphate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
1.7
-
dihydroxyacetone 3-phosphate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
1.8
-
D-glyceraldehyde 3-phosphate mutant S211G, pH 7.5, 25°C Saccharomyces cerevisiae
2.4
-
D-glyceraldehyde 3-phosphate mutant Y208F, pH 7.5, 25°C Saccharomyces cerevisiae
2.9
-
D-glyceraldehyde 3-phosphate mutant Y208A, pH 7.5, 25°C Saccharomyces cerevisiae
3.2
-
dihydroxyacetone 3-phosphate mutant S211G, pH 7.5, 25°C Saccharomyces cerevisiae
3.4
-
D-glyceraldehyde 3-phosphate mutant Y208T, pH 7.5, 25°C Saccharomyces cerevisiae
3.9
-
D-glyceraldehyde 3-phosphate mutant Y208S, pH 7.5, 25°C Saccharomyces cerevisiae
4
-
dihydroxyacetone 3-phosphate mutant Y208T/S211G, pH 7.5, 25°C Saccharomyces cerevisiae
11
-
dihydroxyacetone 3-phosphate mutant Y208T, pH 7.5, 25°C Saccharomyces cerevisiae
12
-
D-glyceraldehyde 3-phosphate mutant S211A, pH 7.5, 25°C Saccharomyces cerevisiae
17
-
dihydroxyacetone 3-phosphate mutant Y208A, pH 7.5, 25°C Saccharomyces cerevisiae
17
-
dihydroxyacetone 3-phosphate mutant Y208F, pH 7.5, 25°C Saccharomyces cerevisiae
25
-
dihydroxyacetone 3-phosphate mutant Y208S, pH 7.5, 25°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P00942
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate
-
Saccharomyces cerevisiae dihydroxyacetone 3-phosphate
-
?
dihydroxyacetone 3-phosphate
-
Saccharomyces cerevisiae D-glyceraldehyde 3-phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5
-
dihydroxyacetone 3-phosphate mutant Y208F, pH 7.5, 25°C Saccharomyces cerevisiae
13
-
D-glyceraldehyde 3-phosphate mutant Y208F, pH 7.5, 25°C Saccharomyces cerevisiae
135
-
dihydroxyacetone 3-phosphate mutant Y208T/S211G, pH 7.5, 25°C Saccharomyces cerevisiae
210
-
dihydroxyacetone 3-phosphate mutant Y208A, pH 7.5, 25°C Saccharomyces cerevisiae
250
-
dihydroxyacetone 3-phosphate mutant Y208S, pH 7.5, 25°C Saccharomyces cerevisiae
520
-
D-glyceraldehyde 3-phosphate mutant Y208T/S211G, pH 7.5, 25°C Saccharomyces cerevisiae
580
-
dihydroxyacetone 3-phosphate mutant Y208T, pH 7.5, 25°C Saccharomyces cerevisiae
740
-
D-glyceraldehyde 3-phosphate mutant Y208A, pH 7.5, 25°C Saccharomyces cerevisiae
810
-
dihydroxyacetone 3-phosphate mutant S211G, pH 7.5, 25°C Saccharomyces cerevisiae
860
-
dihydroxyacetone 3-phosphate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
940
-
D-glyceraldehyde 3-phosphate mutant Y208S, pH 7.5, 25°C Saccharomyces cerevisiae
2800
-
D-glyceraldehyde 3-phosphate mutant S211A, pH 7.5, 25°C Saccharomyces cerevisiae
3700
-
D-glyceraldehyde 3-phosphate mutant Y208T, pH 7.5, 25°C Saccharomyces cerevisiae
7500
-
D-glyceraldehyde 3-phosphate mutant S211G, pH 7.5, 25°C Saccharomyces cerevisiae
8900
-
D-glyceraldehyde 3-phosphate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2 5 arsenate above 25 mM, mutant S211A, pH 7.5, 25°C Saccharomyces cerevisiae
2 5 arsenate above 25 mM, mutant Y208A, pH 7.5, 25°C Saccharomyces cerevisiae
2 5 arsenate above 25 mM, mutant Y208F, pH 7.5, 25°C Saccharomyces cerevisiae
2 5 arsenate above 25 mM, mutant Y208S, pH 7.5, 25°C Saccharomyces cerevisiae
2 5 arsenate above 25 mM, mutant Y208T, pH 7.5, 25°C Saccharomyces cerevisiae
6.5
-
arsenate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
14
-
arsenate mutant S211G, pH 7.5, 25°C Saccharomyces cerevisiae
18
-
arsenate mutant Y208T/S211G, pH 7.5, 25°C Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.26
-
dihydroxyacetone 3-phosphate mutant Y208F, pH 7.5, 25°C Saccharomyces cerevisiae
5.4
-
D-glyceraldehyde 3-phosphate mutant Y208F, pH 7.5, 25°C Saccharomyces cerevisiae
10
-
dihydroxyacetone 3-phosphate mutant S211A, pH 7.5, 25°C Saccharomyces cerevisiae
10
-
dihydroxyacetone 3-phosphate mutant Y208S, pH 7.5, 25°C Saccharomyces cerevisiae
12
-
dihydroxyacetone 3-phosphate mutant Y208A, pH 7.5, 25°C Saccharomyces cerevisiae
34
-
dihydroxyacetone 3-phosphate mutant Y208T/S211G, pH 7.5, 25°C Saccharomyces cerevisiae
53
-
dihydroxyacetone 3-phosphate mutant Y208T, pH 7.5, 25°C Saccharomyces cerevisiae
230
-
D-glyceraldehyde 3-phosphate mutant S211A, pH 7.5, 25°C Saccharomyces cerevisiae
240
-
D-glyceraldehyde 3-phosphate mutant Y208S, pH 7.5, 25°C Saccharomyces cerevisiae
250
-
dihydroxyacetone 3-phosphate mutant S211G, pH 7.5, 25°C Saccharomyces cerevisiae
260
-
D-glyceraldehyde 3-phosphate mutant Y208A, pH 7.5, 25°C Saccharomyces cerevisiae
510
-
dihydroxyacetone 3-phosphate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
730
-
D-glyceraldehyde 3-phosphate mutant Y208T/S211G, pH 7.5, 25°C Saccharomyces cerevisiae
1100
-
D-glyceraldehyde 3-phosphate mutant Y208T, pH 7.5, 25°C Saccharomyces cerevisiae
4200
-
D-glyceraldehyde 3-phosphate mutant S211G, pH 7.5, 25°C Saccharomyces cerevisiae
8900
-
D-glyceraldehyde 3-phosphate wild-type, pH 7.5, 25°C Saccharomyces cerevisiae