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Literature summary for 5.3.1.1 extracted from

  • Zhai, X.; Go, M.K.; ODonoghue, A.C.; Amyes, T.L.; Pegan, S.D.; Wang, Y.; Loria, J.P.; Mesecar, A.D.; Richard, J.P.
    Enzyme architecture the effect of replacement and deletion mutations of loop 6 on catalysis by triosephosphate isomerase (2014), Biochemistry, 53, 3486-3501 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of mutants 170-173del and V167P/W168E Gallus gallus

Protein Variants

Protein Variants Comment Organism
170-173del loop deletion mutant, 800000fold decrease in kcat/Km value Gallus gallus
V167P/W168E loop replacement mutant, 23000fold decrease in kcat/Km value. Mutations result in large displacement of the side chain of E168 from that for W168 in wild-type. Binding of glycerol 3-phosphate results in chemical shift changes for nuclei at the active site that are smaller than those of wild-type Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
Phosphoglycolate
-
Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004
-
D-glyceraldehyde 3-phosphate mutant V167P/W168E, pH 7.5, 25°C Gallus gallus
0.29
-
D-glyceraldehyde 3-phosphate wild-type, pH 7.5, 25°C Gallus gallus
0.59
-
dihydroxyacetone 3-phosphate wild-type, pH 7.5, 25°C Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus P00940
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate
-
Gallus gallus dihydroxyacetone 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
TpI
-
Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
D-glyceraldehyde 3-phosphate mutant 170-173del, pH 7.5, 25°C Gallus gallus
20
-
D-glyceraldehyde 3-phosphate mutant V167P/W168E, pH 7.5, 25°C Gallus gallus
340
-
dihydroxyacetone 3-phosphate wild-type, pH 7.5, 25°C Gallus gallus
3200
-
D-glyceraldehyde 3-phosphate wild-type, pH 7.5, 25°C Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.019
-
Phosphoglycolate wild-type, pH 7.5, 25°C Gallus gallus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
D-glyceraldehyde 3-phosphate mutant 170-173del, pH 7.5, 25°C Gallus gallus
0.47
-
D-glyceraldehyde 3-phosphate mutant V167P/W168E, pH 7.5, 25°C Gallus gallus
580
-
dihydroxyacetone 3-phosphate wild-type, pH 7.5, 25°C Gallus gallus
11000
-
D-glyceraldehyde 3-phosphate wild-type, pH 7.5, 25°C Gallus gallus