Crystallization (Comment) | Organism |
---|---|
structures of mutants 170-173del and V167P/W168E | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
170-173del | loop deletion mutant, 800000fold decrease in kcat/Km value | Gallus gallus |
V167P/W168E | loop replacement mutant, 23000fold decrease in kcat/Km value. Mutations result in large displacement of the side chain of E168 from that for W168 in wild-type. Binding of glycerol 3-phosphate results in chemical shift changes for nuclei at the active site that are smaller than those of wild-type | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phosphoglycolate | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
D-glyceraldehyde 3-phosphate | mutant V167P/W168E, pH 7.5, 25°C | Gallus gallus | |
0.29 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Gallus gallus | |
0.59 | - |
dihydroxyacetone 3-phosphate | wild-type, pH 7.5, 25°C | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P00940 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Gallus gallus | dihydroxyacetone 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TpI | - |
Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
D-glyceraldehyde 3-phosphate | mutant 170-173del, pH 7.5, 25°C | Gallus gallus | |
20 | - |
D-glyceraldehyde 3-phosphate | mutant V167P/W168E, pH 7.5, 25°C | Gallus gallus | |
340 | - |
dihydroxyacetone 3-phosphate | wild-type, pH 7.5, 25°C | Gallus gallus | |
3200 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
Phosphoglycolate | wild-type, pH 7.5, 25°C | Gallus gallus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
D-glyceraldehyde 3-phosphate | mutant 170-173del, pH 7.5, 25°C | Gallus gallus | |
0.47 | - |
D-glyceraldehyde 3-phosphate | mutant V167P/W168E, pH 7.5, 25°C | Gallus gallus | |
580 | - |
dihydroxyacetone 3-phosphate | wild-type, pH 7.5, 25°C | Gallus gallus | |
11000 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Gallus gallus |