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Literature summary for 5.3.1.1 extracted from

  • Knobeloch, D.; Schmidt, A.; Scheerer, P.; Krauss, N.; Wessner, H.; Scholz, C.h.; Kuettner, G.; von Rintelen, T.; Wessel, A.; Hoehne, W.
    A coleopteran triosephosphate isomerase: X-ray structure and phylogenetic impact of insect sequences (2010), Insect Mol. Biol., 19, 35-48.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged TIM in Escherichia coli cytoplasm in a natively folded, enzymatically active form Tenebrio molitor

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant C-terminally His6-tagged enzyme, sitting drop vapour diffusion method, room temperature, 0.003 ml of 18 mg/ml protein in 10 mM MES buffer, pH 6.5, and 15 mM NaCl, is mixed with 0.003 ml of reservoir solution containing 18% PEG MME 2000 as precipitant, X-ray diffraction structure determination and analysis at 2.0 A resolution Tenebrio molitor

Inhibitors

Inhibitors Comment Organism Structure
glycerol 1-phosphate
-
Tenebrio molitor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.84
-
D-glyceraldehyde 3-phosphate pH 7.0, 25°C Tenebrio molitor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Glyceraldehyde 3-phosphate Tenebrio molitor
-
Glycerone phosphate
-
?

Organism

Organism UniProt Comment Textmining
Tenebrio molitor Q8MPF2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged TIM from Escherichia coli by metal affinity chromatography Tenebrio molitor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glyceraldehyde 3-phosphate
-
Tenebrio molitor Glycerone phosphate
-
?

Subunits

Subunits Comment Organism
dimer gel filtration and SDS-PAGE, crystal structure, overview Tenebrio molitor
More structure analysis and loop interactions of TIM, overview Tenebrio molitor

Synonyms

Synonyms Comment Organism
TIM
-
Tenebrio molitor
Triosephosphate isomerase
-
Tenebrio molitor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Tenebrio molitor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4300
-
D-glyceraldehyde 3-phosphate pH 7.0, 25°C Tenebrio molitor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 8.7
-
Tenebrio molitor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8
-
glycerol 1-phosphate
-
Tenebrio molitor

General Information

General Information Comment Organism
metabolism triosephosphate isomerase is a ubiquitously distributed enzyme of the glycolysis pathway Tenebrio molitor

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5119
-
D-glyceraldehyde 3-phosphate pH 7.0, 25°C Tenebrio molitor