Literature summary for 5.3.1.1 extracted from

Espinoza-Fonseca, L.M.; Wong-Ramirez, C.; Trujillo-Ferrara, J.G.
Tyr74 is essential for the formation, stability and function of Plasmodium falciparum triosephosphate isomerase dimer (2010), Arch. Biochem. Biophys., 494, 46-57.

Search for more literature for 5.3.1.1

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure analysis, overview	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
Y74G	site-directed mutagenesis, the mutation Tyr74Gly significantly reduces the stability of the dimer, mutation-induced alteration in the backbone conformation of Lys12, structure comparison to the wild-type enzyme	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-Glyceraldehyde 3-phosphate	Plasmodium falciparum	-	Glycerone phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q07412	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glyceraldehyde 3-phosphate	-	Plasmodium falciparum	Glycerone phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	TIM is functional only as a homodimer, the interface Cys13 plays a major role in the stability of the dimer, Cys13 forms favorable interactions with loop 3 and Lys12. Structurally conserved Tyr74 may be essential for the stability, it is necessary to preserve the collective motions in the dimer that contribute to the catalytic efficiency of the TIM dimer. Tyr74 is a ready-made recognition motif for TIM homodimerization	Plasmodium falciparum
More	simulation of the dynamics of monomeric TIM subunit A, molecular dynamics simulations, disulfide cross-linking at the interface is required for stability, the absence of a disulfide bond between Cys13 and Cys74 produces a dramatic shift in the conformation of Lys12, overview	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
TIM	-	Plasmodium falciparum
Triosephosphate isomerase	-	Plasmodium falciparum

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Release 2023.1 (January 2023)