Cloned (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
- |
Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Geobacillus stearothermophilus |
hanging drop vapor diffusion method | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
H12N | mutant enzyme with decreased thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 64°C compared to 68°C for the wild-type enzyme | Geobacillus stearothermophilus |
H12N/K13G | mutant enzyme shows increases thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 96°C compared to 94°C of the wild-type enzyme | Thermotoga maritima |
H12N/K13G | mutant enzyme with decreased thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 37°C compared to 68°C for the wild-type enzyme | Geobacillus stearothermophilus |
H13G | mutant enzyme with decreased thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 51°C compared to 68°C for the wild-type enzyme | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzyme H12N/K13G | Thermotoga maritima |
wild-type and mutant enzymes H12N, H13G and H12N/K13G | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Lys13 plays a crucial role in the functional adaptation of the thermophilic enzyme to high temperatures | Geobacillus stearothermophilus |
37 | - |
half-life of mutant enzyme H12N/K13G is 11.5 min | Geobacillus stearothermophilus |
51 | - |
half-life of mutant enzyme H13G is 11.5 min | Geobacillus stearothermophilus |
64 | - |
half-life of mutant enzyme H12N is 11.5 min | Geobacillus stearothermophilus |
68 | - |
half-life of wild-type recombinant enzyme is 11.5 min | Geobacillus stearothermophilus |
94 | - |
half-life of wild-type recombinant enzyme is 11.5 min | Thermotoga maritima |
96 | - |
half-life of mutant enzyme H12N/K13G is 11.5 min | Thermotoga maritima |