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Literature summary for 5.2.1.8 extracted from
- Identification and functional analysis of a novel parvulin-type peptidyl-prolyl isomerase from Gossypium hirsutum (2014), Plant Physiol. Biochem., 76, 58-66.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PPI, phylogenetic analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), expression of GhPPI-GFP fusion enzyme in onion epidermal cell nuclei from pBI121-GFP using Agrobacterium tumefaciens strain LBA4404 for transfection | Gossypium hirsutum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C138A | site-directed mutagenesis, the mutant shows 93% of wild-type activity | Gossypium hirsutum |
| C41A | site-directed mutagenesis, the mutant shows 104% of wild-type activity | Gossypium hirsutum |
| C51A | site-directed mutagenesis, the mutant shows 93% of wild-type activity | Gossypium hirsutum |
| C90A | site-directed mutagenesis, the mutant shows 35% of wild-type activity | Gossypium hirsutum |
| F111A | site-directed mutagenesis, the mutant shows 30% of wild-type activity | Gossypium hirsutum |
| F111Y | site-directed mutagenesis, the mutant shows 20% of wild-type activity | Gossypium hirsutum |
| H131A | site-directed mutagenesis, the mutant shows 19% of wild-type activity | Gossypium hirsutum |
| H134A | site-directed mutagenesis, the mutant shows 30% of wild-type activity | Gossypium hirsutum |
| H48A | site-directed mutagenesis, the mutant shows 7.8% of wild-type activity | Gossypium hirsutum |
| M107A | site-directed mutagenesis, the mutant shows 73% of wild-type activity | Gossypium hirsutum |
| T130A | site-directed mutagenesis, the mutant shows 68% of wild-type activity | Gossypium hirsutum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Gossypium hirsutum | 5634 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gossypium hirsutum | Q4KNC9 | - |
- |
| Gossypium hirsutum ZM3 | Q4KNC9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Gossypium hirsutum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | purified recombinant GhPPI accelerates the initial velocity of the cis-trans conversion of peptidyl-prolyl bonds of a tetrapeptide in a GhPPI concentration-dependent manner. Recombinant GhPPI also suppresses protein aggregation under denaturing conditions using guanidine hydrochloride, suggesting an additional chaperone activity | Gossypium hirsutum | ? | - |
? |  |
| additional information | purified recombinant GhPPI accelerates the initial velocity of the cis-trans conversion of peptidyl-prolyl bonds of a tetrapeptide in a GhPPI concentration-dependent manner. Recombinant GhPPI also suppresses protein aggregation under denaturing conditions using guanidine hydrochloride, suggesting an additional chaperone activity | Gossypium hirsutum ZM3 | ? | - |
? | |
| N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | - |
Gossypium hirsutum | ? | - |
? | |
| N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | - |
Gossypium hirsutum ZM3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | three-dimensional structure of GhPPI by homology modeling, molecular docking, structure of the substrate binding site of GhPPI, overview | Gossypium hirsutum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| parvulin-type peptidyl-prolyl isomerase | - |
Gossypium hirsutum |
| peptidyl-prolyl cis/trans isomerase | - |
Gossypium hirsutum |
| PPIase | - |
Gossypium hirsutum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gossypium hirsutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Gossypium hirsutum |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Gossypium hirsutum | mRNA accumulation of GhPPI is induced by salt stress | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the parvulin family of peptidyl-prolyl cis/trans isomerases, PPIases | Gossypium hirsutum |
| additional information | three-dimensional structure of GhPPI by homology modeling, molecular docking, structure of the substrate binding site of GhPPI, overview | Gossypium hirsutum |
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Release 2023.1 (January 2023)
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