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Literature summary for 5.2.1.8 extracted from

  • Iida, T.; Iwabuchi, T.; Ideno, A.; Suzuki, S.; Maruyama, T.
    FK506-binding protein-type peptidyl-prolyl cis-trans isomerase from a halophilic archaeum, Halobacterium cutirubrum (2000), Gene, 256, 319-326.
    View publication on PubMed
Search for more literature for 5.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a fusion protein with glutathione S-transferase in Escherichia coli, and the N-terminal glutathione S-transferase portion is removed by protease digestion Halobacterium salinarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33345
-
 6 * 33345, calculated from sequence Halobacterium salinarum
43000
-
 6 * 43000, recombinant enzyme, SDS-PAGE Halobacterium salinarum
200000
-
 gel filtration Halobacterium salinarum

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum Q9P9H4
-
-
Halobacterium salinarum DSM 669 Q9P9H4
-
-

Purification (Commentary)

Purification (Comment) Organism
recpmbinant enzyme Halobacterium salinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Ala-(cis)-Pro-Phe-p-nitroanilide
-
 Halobacterium salinarum N-succinyl-Ala-Ala-(trans)-Pro-Phe-p-nitroanilide
-
 ?
N-succinyl-Ala-Ala-(cis)-Pro-Phe-p-nitroanilide
-
 Halobacterium salinarum DSM 669 N-succinyl-Ala-Ala-(trans)-Pro-Phe-p-nitroanilide
-
 ?
N-succinyl-Ala-Leu-(cis)-Pro-Phe-p-nitroanilide
-
 Halobacterium salinarum N-succinyl-Ala-Leu-(trans)-Pro-Phe-p-nitroanilide
-
 ?
N-succinyl-Ala-Leu-(cis)-Pro-Phe-p-nitroanilide
-
 Halobacterium salinarum DSM 669 N-succinyl-Ala-Leu-(trans)-Pro-Phe-p-nitroanilide
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 33345, calculated from sequence Halobacterium salinarum
hexamer 6 * 43000, recombinant enzyme, SDS-PAGE Halobacterium salinarum

Synonyms

Synonyms Comment Organism
FKBP33
-
 Halobacterium salinarum
PPIase
-
 Halobacterium salinarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 assay at Halobacterium salinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Halobacterium salinarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.78
-
 N-succinyl-Ala-Leu-(cis)-Pro-Phe-p-nitroanilide pH 7.8, 15°C Halobacterium salinarum