Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | stimulation | Homo sapiens | |
ATP | stimulation | Saccharomyces cerevisiae | |
ATP | at least 3fold stimulation, excess of ATP over Mg2+ is inhibitory | Saccharomyces cerevisiae | |
ATP | at least 3fold stimulation, excess of ATP over Mg2+ is inhibitory | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
D205G | essential for petidyl-prolyl cis/trans isomerase activity and activation of PP2A phosphatase | Saccharomyces cerevisiae |
D205G | D205 is required for both peptidylprolyl isomerase activity and protein phosphatase 2A activation | Saccharomyces cerevisiae |
DELTA208-213 | almost no enzymic activity, 400fold less activity in the activation reaction of PP2A phosphatase | Oryctolagus cuniculus |
DELTA208-213 | dramatic reduction of peptidylprolyl isomerase activity and 400fold reduction of protein phosphatase 2A activation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | at least 3fold stimulation, excess of ATP over Mg2+ is inhibitory | Oryctolagus cuniculus | |
ATP | at least 3fold stimulation, excess of ATP over Mg2+ is inhibitory | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulation | Homo sapiens | |
Mg2+ | stimulation | Saccharomyces cerevisiae | |
Mg2+ | at least 3fold stimulation | Saccharomyces cerevisiae | |
Mg2+ | at least 3fold stimulation | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
PP2A phosphatase with cis-P190 | Saccharomyces cerevisiae | - |
PP2A phosphatase with trans-P190 | - |
? | |
PP2A phosphatase with cis-P190 | Oryctolagus cuniculus | - |
PP2A phosphatase with trans-P190 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oryctolagus cuniculus | - |
recombinant wild-type enzyme and a His-tagged version | - |
Saccharomyces cerevisiae | - |
isoforms Ypa1, Ypa2 | - |
Saccharomyces cerevisiae | - |
isoform Ypa1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(trans)-Pro190 of protein phosphatase 2A | - |
Homo sapiens | (cis)-Pro190 of protein phosphatase 2A | - |
? | |
(trans)-Pro190 of protein phosphatase 2A | - |
Saccharomyces cerevisiae | (cis)-Pro190 of protein phosphatase 2A | - |
? | |
PP2A phosphatase with cis-P190 | - |
Saccharomyces cerevisiae | PP2A phosphatase with trans-P190 | - |
? | |
PP2A phosphatase with cis-P190 | - |
Oryctolagus cuniculus | PP2A phosphatase with trans-P190 | - |
? | |
Suc-Ala-Ala-(trans)-Pro-Lys-p-nitroanilide | - |
Homo sapiens | Suc-Ala-Ala-(cis)-Pro-Lys-p-nitroanilide | - |
? | |
Suc-Ala-Ala-(trans)-Pro-Lys-p-nitroanilide | - |
Saccharomyces cerevisiae | Suc-Ala-Ala-(cis)-Pro-Lys-p-nitroanilide | - |
? | |
Suc-Ala-Ala-(trans)-Pro-Phe-methylcoumarylamide | - |
Homo sapiens | Suc-Ala-Ala-(cis)-Pro-Phe-methylcoumarylamide | - |
? | |
Suc-Ala-Ala-(trans)-Pro-Phe-methylcoumarylamide | - |
Saccharomyces cerevisiae | Suc-Ala-Ala-(cis)-Pro-Phe-methylcoumarylamide | - |
? | |
Suc-Ala-Ala-(trans)-Pro-Phe-p-nitroanilide | - |
Homo sapiens | Suc-Ala-Ala-(cis)-Pro-Phe-p-nitroanilide | - |
? | |
Suc-Ala-Ala-(trans)-Pro-Phe-p-nitroanilide | - |
Saccharomyces cerevisiae | Suc-Ala-Ala-(cis)-Pro-Phe-p-nitroanilide | - |
? | |
Suc-Ala-Glu-(trans)-Pro-Phe-p-nitroanilide | - |
Homo sapiens | Suc-Ala-Glu-(cis)-Pro-Phe-p-nitroanilide | - |
? | |
Suc-Ala-Glu-(trans)-Pro-Phe-p-nitroanilide | - |
Saccharomyces cerevisiae | Suc-Ala-Glu-(cis)-Pro-Phe-p-nitroanilide | - |
? | |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | - |
Saccharomyces cerevisiae | succinyl-Ala-Ala-(trans)-Pro-Lys + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | - |
Oryctolagus cuniculus | succinyl-Ala-Ala-(trans)-Pro-Lys + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide | - |
Saccharomyces cerevisiae | succinyl-Ala-Ala-(trans)-Pro-Phe + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide | - |
Oryctolagus cuniculus | succinyl-Ala-Ala-(trans)-Pro-Phe + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PP2A phosphatase activator | - |
Oryctolagus cuniculus |
protein phosphatase 2A phosphatase activator | - |
Homo sapiens |
PtpA | - |
Homo sapiens |
PtpA | - |
Oryctolagus cuniculus |
Ypa | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | 20°C, pH 7.4, mutant DELTA208-213 | Oryctolagus cuniculus | |
0.008 | - |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | 20°C, pH 7.4, mutant DELTA208-213, presence of 1 mM MgATP | Oryctolagus cuniculus | |
0.011 | - |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | 20°C, pH 7.4, mutant D205G of Ypa1 | Saccharomyces cerevisiae | |
0.016 | - |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | 20°C, pH 7.4, mutant D205G of Ypa1, presence of 1 mM MgATP | Saccharomyces cerevisiae | |
0.02 | - |
succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide | 20°C, pH 7.4, wild-type Ypa1 | Saccharomyces cerevisiae | |
0.03 | - |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | 20°C, pH 7.4, wild-type | Oryctolagus cuniculus | |
0.03 | - |
succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide | 20°C, pH 7.4, wild-type | Oryctolagus cuniculus | |
0.03 | - |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | 20°C, pH 7.4, wild-type Ypa2 | Saccharomyces cerevisiae | |
0.05 | - |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | 20°C, pH 7.4, wild-type Ypa2, presence of 1 mM MgATP | Saccharomyces cerevisiae | |
0.07 | - |
succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide | 20°C, pH 7.4, wild-type Ypa1, presence of 1 mM MgATP | Saccharomyces cerevisiae | |
0.1 | - |
succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide | 20°C, pH 7.4, wild-type, presence of 1 mM MgATP | Oryctolagus cuniculus | |
0.19 | - |
succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide | 20°C, pH 7.4, wild-type, presence of 1 mM MgATP | Oryctolagus cuniculus |