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Literature summary for 5.2.1.8 extracted from
- Biochemical and genetic characterization of an FK506-sensitive peptidyl prolyl cis-trans isomerase from a thermophilic archaeon, Methanococcus thermolithotrophicus (1998), J. Bacteriol., 180, 388-394.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Methanothermococcus thermolithotrophicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyclosporin A | no inhibition by cyclosporins | Methanothermococcus thermolithotrophicus |  |
| FK506 | IC50: 250 nM | Methanothermococcus thermolithotrophicus | |
| additional information | no inhibition by cyclosporine, even at 0.01 mM | Methanothermococcus thermolithotrophicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
x * 16000, SDS-PAGE | Methanothermococcus thermolithotrophicus |
| 42000 | - |
gel filtration | Methanothermococcus thermolithotrophicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermococcus thermolithotrophicus | - |
- |
- |
| Methanothermococcus thermolithotrophicus | O52980 | DSM2095 | - |
| Methanothermococcus thermolithotrophicus DSM 2095 | O52980 | DSM2095 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermococcus thermolithotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Methanothermococcus thermolithotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide | - |
Methanothermococcus thermolithotrophicus | N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide | - |
r | |
| N-succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide | - |
Methanothermococcus thermolithotrophicus DSM 2095 | N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide | - |
r | |
| N-succinyl-Ala-Leu-(trans)-Pro-Phe-4-nitroanilide | - |
Methanothermococcus thermolithotrophicus | N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide | - |
r | |
| N-succinyl-Ala-Leu-(trans)-Pro-Phe-4-nitroanilide | - |
Methanothermococcus thermolithotrophicus DSM 2095 | N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide | - |
r | |
| succinyl-Ala-Ala-Pro-Phe 4-nitroanilide | - |
Methanothermococcus thermolithotrophicus | succinyl-Ala-Ala-(trans)-Pro-Phe 4-nitroanilide | - |
? | |
| Succinyl-Ala-Leu-(cis)-Pro-Phe 4-nitroanilide | - |
Methanothermococcus thermolithotrophicus | Succinyl-Ala-Leu-(trans)-Pro-Phe 4-nitroanilide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 16000, SDS-PAGE | Methanothermococcus thermolithotrophicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MtFK | - |
Methanothermococcus thermolithotrophicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
half-life: 90 min | Methanothermococcus thermolithotrophicus |
| 100 | - |
half-life: 30 min | Methanothermococcus thermolithotrophicus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
IC50: 250 nM | Methanothermococcus thermolithotrophicus | FK506 | |
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