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Literature summary for 5.2.1.2 extracted from

  • Board, P.G.; Taylor, M.C.; Coggan, M.; Parker, M.W.; Lantum, H.B.; Anders, M.W.
    Clarification of the role of key active site residues of glutathione transferase zeta/maleylacetoacetate isomerase by a new spectrophotometric technique (2003), Biochem. J., 374, 731-737.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.2.1.2

Protein Variants

Protein Variants Comment Organism
C16A the ratio of turnover number to KM-value for (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid is 2.1fold decreased compared to the wild-type enzyme, the ratio of turnover number to KM-value for 4-maleylacetoacetate is 1.6fold increased compared to wild-type enzyme Homo sapiens
R175A the ratio of turnover number to KM-value for (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid is 1.5fold decreased compared to the wild-type enzyme, the ratio of turnover number to KM-value for 4-maleylacetoacetate is 6.8fold decreased compared to wild-type enzyme Homo sapiens
R175K the ratio of turnover number to KM-value for (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid is 1.8fold increased compared to the wild-type enzyme, the ratio of turnover number to KM-value for 4-maleylacetoacetate is 1.3fold increased compared to wild-type enzyme Homo sapiens
S14A mutant enzyme shows no activity with {(+/-)-2-bromo-3-(4-nitrophenyl)propionic acid} or 4-maleylacetoacetate Homo sapiens
S15A the ratio of turnover number to KM-value for (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid is 2.3fold decreased compared to the wild-type enzyme, the ratio of turnover number to KM-value for 4-maleylacetoacetate is 11.8fold decreased compared to wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175A Homo sapiens
0.027
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
0.029
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1d-1d Homo sapiens
0.044
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1c-1c Homo sapiens
0.068
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme C165A Homo sapiens
0.096
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1b-1b Homo sapiens
0.128
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1a-1a Homo sapiens
0.223
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
0.319
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme S15A Homo sapiens
0.368
-
 4-Maleylacetoacetate pH 7.6, 25°C, wild-type enzyme Homo sapiens
0.417
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme C16A Homo sapiens
0.64
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme S15A Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme plays an important role in the metabolism of tyrosine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 spectrophotometric assay with (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-2-bromo-3-(4-nitrophenyl)propionic acid + glutathione it is proposed that the charged side chain of Arg-175 forms a salt bridge with the carboxylate of the alpha-halo acid substrates Homo sapiens 2-(glutathion-S-yl)-3-(4-nitrophenyl)propanoic acid
-
 ?
4-maleylacetoacetate
-
 Homo sapiens 4-fumarylacetoacetate
-
 ?
additional information the enzyme plays an important role in the metabolism of tyrosine Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
glutathione S-transferase Zeta 1-1
-
 Homo sapiens
GSTZ1-1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175A Homo sapiens
0.55
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1d-1d Homo sapiens
0.59
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1c-1c Homo sapiens
0.61
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
0.82
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1b-1b Homo sapiens
1
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme S15A Homo sapiens
1.3
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1a-1a Homo sapiens
2.1
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme S15A Homo sapiens
2.55
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme C16A Homo sapiens
6.08
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1b-1b Homo sapiens
6.08
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, enzyme hGSTZ1c-1c Homo sapiens
6.08
-
 (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
72.6
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme S15A Homo sapiens
141
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme C165A Homo sapiens
149
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme R175A Homo sapiens
368
-
 4-Maleylacetoacetate pH 7.6, 25°C, mutant enzyme R175K Homo sapiens
464
-
 4-Maleylacetoacetate pH 7.6, 25°C, wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5 11 reaction with (+/-)-2-bromo-3-(4-nitrophenyl)propionic acid and glutathione Homo sapiens