Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Serratia marcescens |
Protein Variants | Comment | Organism |
---|---|---|
M138C | mutant enzyme shows 90% of the activity of the wild-type enzyme | Serratia marcescens |
M18C | mutant enzyme shows 30% of the activity of the wild-type enzyme | Serratia marcescens |
M201C | mutant enzyme shows 90% of the activity of the wild-type enzyme | Serratia marcescens |
M46C | mutant enzyme shows 30% of the activity of the wild-type enzyme | Serratia marcescens |
M57C | mutant enzyme shows 40% of the activity of the wild-type enzyme | Serratia marcescens |
M85C | mutant enzyme shows 90% of the activity of the wild-type enzyme | Serratia marcescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | 1 mM, complete inhibition, Met201 is one of the targeted sites for the inactivation by chemical oxidation | Serratia marcescens | |
iodoacetamide | 1 mM, 72% inhibition | Serratia marcescens | |
N-bromosuccinimide | 1 mM, complete inhibition | Serratia marcescens | |
N-Chlorosuccinimide | 1 mM, complete inhibition | Serratia marcescens | |
p-toluenesulfonchloramide | 1 mM, complete inhibition | Serratia marcescens | |
PCMB | 0.1 mM, complete inhibition | Serratia marcescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26950 | - |
x * 26950, calculation from nucleotide sequence | Serratia marcescens |
28000 | - |
x * 28000, SDS-PAGE | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Serratia marcescens | - |
IFO3736 | - |
Serratia marcescens IFO3736 | - |
IFO3736 | - |
Purification (Comment) | Organism |
---|---|
- |
Serratia marcescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Maleate | - |
Alcaligenes faecalis | Fumarate | - |
? | |
Maleate | - |
Geobacillus stearothermophilus | Fumarate | - |
? | |
Maleate | - |
Serratia marcescens | Fumarate | - |
? | |
Maleate | - |
Serratia marcescens IFO3736 | Fumarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Serratia marcescens |
? | x * 26950, calculation from nucleotide sequence | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
MaiA | - |
Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Alcaligenes faecalis |
45 | - |
- |
Serratia marcescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | increase of activity from 30°C to 70°C | Geobacillus stearothermophilus |
30 | 60 | 30°C: about 60% of maximal activity, 60°C: about 40% of maximal activity | Alcaligenes faecalis |
30 | 60 | 30°C: about 70% of maximal activity, 60°C: about 70% of maximal activity | Serratia marcescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, stable | Alcaligenes faecalis |
45 | - |
30 min, about 10% loss of activity | Alcaligenes faecalis |
50 | - |
30 min, about 45% loss of activity | Alcaligenes faecalis |
53 | - |
30 min, complete inactivation | Alcaligenes faecalis |
57 | - |
30 min, stable below | Geobacillus stearothermophilus |
60 | - |
30 min, about 25% loss of activity | Geobacillus stearothermophilus |
60 | - |
30 min, retains almost 60% of the initial activity | Serratia marcescens |