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Literature summary for 5.1.99.4 extracted from

  • Darley, D.J.; Butler, D.S.; Prideaux, S.J.; Thornton, T.W.; Wilson, A.D.; Woodman, T.J.; Threadgill, M.D.; Lloyd, M.D.
    Synthesis and use of isotope-labelled substrates for a mechanistic study on human alpha-methylacyl-CoA racemase 1A (AMACR; P504S) (2009), Org. Biomol. Chem., 7, 543-552.
    View publication on PubMed
Search for more literature for 5.1.99.4

Application

Application Comment Organism
diagnostics biomarker for cancer in prostate, breast, colon, renal and other cancer cells Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
AMACR 1A, the major splice variant, is expressed as a His-tag protein in Escherichia coli cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 (S)-2-methyldecanoyl-CoA recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2 Homo sapiens
1.2
-
 (R)-2-methyldecanoyl-CoA recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Homo sapiens 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 determined by SDS-PAGE Homo sapiens
47150
-
 calculated fpor His-tagged protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UHK6
-
-

Purification (Commentary)

Purification (Comment) Organism
purification by affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68.4
-
 substrate is (R)-2-methyldecanoyl-CoA, recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2 Homo sapiens
77.5
-
 substrate is (S)-2-methyldecanoyl-CoA, recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-methyldecanoyl-CoA
-
 Homo sapiens (S)-2-methyldecanoyl-CoA
-
 r
(R)-2-methyltetradecanoyl-CoA
-
 Homo sapiens (S)-2-methyltetradecanoyl-CoA
-
 r
(R)-ibuprofenoyl-CoA
-
 Homo sapiens (S)-ibuprofenoyl-CoA
-
 r
(R)-pristanoyl-CoA
-
 Homo sapiens (S)-pristanoyl-CoA
-
 r
S-[(2R)-2-methyl-6-[(3alpha,5beta,7alpha)-3,7,12-trihydroxy-10-methylgonan-17-yl]heptanoyl]-CoA
-
 Homo sapiens S-[(2S)-2-methyl-6-[(3alpha,5beta,7alpha)-3,7,12-trihydroxy-10-methylgonan-17-yl]heptanoyl]-CoA
-
 r

Synonyms

Synonyms Comment Organism
alpha-methylacyl-CoA racemase 1A
-
 Homo sapiens
AMACR
-
 Homo sapiens
P504S
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
53.8
-
 (R)-2-methyldecanoyl-CoA recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2 Homo sapiens
60.9
-
 (S)-2-methyldecanoyl-CoA recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens enzyme is over-expressed in prostate and other cancers up

General Information

General Information Comment Organism
metabolism beta-oxidation of branched-chain fatty acids, important enzyme for the metabolism of branched-chain lipids and drugs Homo sapiens
physiological function alpha-methylacyl-CoA racemase catalyses the inversion of configuration at the 2-position of fatty acyl-CoA esters from 2R to 2S and controls entry of metabolites into peroxisomal beta-oxidation Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
44.8
-
 (R)-2-methyldecanoyl-CoA recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2 Homo sapiens
50.75
-
 (S)-2-methyldecanoyl-CoA recombinant enzyme, 50 mM NaH2PO4-NaOH, pH 7.2 Homo sapiens