Any feedback?
Literature summary for 5.1.99.3 extracted from
- Allantoin racemase: a new enzyme from Pseudomonas species (1975), Biochim. Biophys. Acta, 391, 240-248.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | preincubation for 30 min at 30 C stimulates | Comamonas testosteroni |  |
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing inactivates | Comamonas testosteroni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Comamonas testosteroni | |
| 5-Aminohydantoin | - |
Comamonas testosteroni | |
| Co2+ | 0.8 mM | Comamonas testosteroni | |
| hydantoin | - |
Comamonas testosteroni | |
| Hydantoin of N-carbamoyl-L-methionine | - |
Comamonas testosteroni | |
| iodoacetate | - |
Comamonas testosteroni | |
| Mn2+ | 0.8 mM | Comamonas testosteroni | |
| NH2OH | - |
Comamonas testosteroni | |
| Zn2+ | 0.8 mM | Comamonas testosteroni | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Comamonas testosteroni |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for several weeks | Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)(+)-Allantoin | - |
Pseudomonas fluorescens | (R)(-)-Allantoin | - |
? | |
| (S)(+)-Allantoin | - |
Pseudomonas putida | (R)(-)-Allantoin | - |
? | |
| (S)(+)-Allantoin | r | Comamonas testosteroni | (R)(-)-Allantoin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Comamonas testosteroni |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 35% of maximal activity, 30°C: 67% of maximal activity, 60°C: about 85% of maximal activity | Comamonas testosteroni |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
15 min, pH 6.5-7, stable up to | Comamonas testosteroni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.2 | - |
Comamonas testosteroni |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | up to 70°C, 15 min, stable | Comamonas testosteroni |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
