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Literature summary for 5.1.99.1 extracted from

  • Han, Y.; Hawkins, A.S.; Adams, M.W.; Kelly, R.M.
    Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle (2012), Appl. Environ. Microbiol., 78, 6194-6202.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.99.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Metallosphaera sedula
gene Msed_0639, DNA and amino acid sequence determination, phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) Metallosphaera sedula

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Metallosphaera sedula
Co2+ the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+) Metallosphaera sedula
Mg2+ activates Metallosphaera sedula
Mg2+ the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+) Metallosphaera sedula
Ni2+ activates Metallosphaera sedula
Ni2+ the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+) Metallosphaera sedula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 2 * 20000, SDS-PAGE Metallosphaera sedula
20000
-
 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE Metallosphaera sedula
32100
-
 gel filtration Metallosphaera sedula
32100
-
 recombinant His-tagged enzyme, gel filtration Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-methylmalonyl-CoA Metallosphaera sedula
-
 (R)-methylmalonyl-CoA
-
 ?
(S)-methylmalonyl-CoA Metallosphaera sedula the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway (R)-methylmalonyl-CoA
-
 ?
(S)-methylmalonyl-CoA Metallosphaera sedula DSM 5348
-
 (R)-methylmalonyl-CoA
-
 ?
(S)-methylmalonyl-CoA Metallosphaera sedula DSM 5348 the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway (R)-methylmalonyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YEG2
-
-
Metallosphaera sedula DSM 5348 A4YEG2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Metallosphaera sedula
recombinant N-terminally His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography Metallosphaera sedula

Source Tissue

Source Tissue Comment Organism Textmining
additional information transcriptomic analyses of Metallosphaera sedula grown under autotrophic versus heterotrophic conditions, overview. MCE expression is significantly upregulated under autotrophic compared to heterotrophic growth conditions Metallosphaera sedula
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
218
-
 75°C, pH not specified in the publication Metallosphaera sedula
218
-
 purified recombinant enzyme, pH 7.0, 75°C Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-methylmalonyl-CoA
-
 Metallosphaera sedula (R)-methylmalonyl-CoA
-
 ?
(S)-methylmalonyl-CoA the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway Metallosphaera sedula (R)-methylmalonyl-CoA
-
 ?
(S)-methylmalonyl-CoA
-
 Metallosphaera sedula DSM 5348 (R)-methylmalonyl-CoA
-
 ?
(S)-methylmalonyl-CoA the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway Metallosphaera sedula DSM 5348 (R)-methylmalonyl-CoA
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 20000, SDS-PAGE Metallosphaera sedula
homodimer 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE Metallosphaera sedula
More the putative three-dimensional structure of the enzyme is modeled using hypothetical protein BH1468, PDB ID 3OA4 Metallosphaera sedula

Synonyms

Synonyms Comment Organism
MCE
-
 Metallosphaera sedula
methylmalonyl-CoA epimerase
-
 Metallosphaera sedula
methylmalonyl-coenzyme A epimerase
-
 Metallosphaera sedula
Msed_0639 locus name Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
73
-
-
 Metallosphaera sedula
75
-
 assay at Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Metallosphaera sedula

Expression

Organism Comment Expression
Metallosphaera sedula expression of the gene is significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO2 fixation up
Metallosphaera sedula the enzyme expression is significantly upregulated under autotrophic compared to heterotrophic growth conditions up

General Information

General Information Comment Organism
physiological function methylmalonyl-CoA epimerase and methylmalonyl-CoA mutase are responsible for producing succinyl-CoA from (S)-methylmalonyl-CoA. Succinyl-CoA is an intermediate connecting the 3-hydroxypropionate/4-hydroxybutyrate cycle and general carbon metabolism. Methylmalonyl-CoA epimerase and methylmalonyl-CoA mutase may also be involved in degradation of propionate and branched-chain amino acids (valine, isoleucine, methionine). The enzyme has a role in CO2 fixation Metallosphaera sedula
physiological function the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway Metallosphaera sedula