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Literature summary for 5.1.99.1 extracted from

  • Shi, L.; Gao, P.; Yan, X.; Liang, D.
    Crystal structure of a putative methylmalonyl-coenzyme a epimerase from Thermoanaerobacter tengcongensis at 2.0 Å resolution (2009), Proteins, 77, 994-999.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
recombinant protein is expressed in BL21(DE3) Escherichia coli cells Caldanaerobacter subterraneus subsp. tengcongensis

Crystallization (Commentary)

Crystallization (Comment) Organism
at 2.0 A resolutionm, crystals of recombinant enzyme are grown at 20°C using the hanging-drop, vapor-diffusion method, drops consisted of 2 microl of protein solution and 2 microl of mother liquor (0.1 M citric acid, pH 2.6, 1.6 M (NH4)2SO4), crystals are obtained after 7-8 days growth, native and derivative crystals are soaked in 2 M Li2SO4 Caldanaerobacter subterraneus subsp. tengcongensis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme is metal-dependent enzyme, in the active site, metal binding site binds the coenzyme Co2+ Caldanaerobacter subterraneus subsp. tengcongensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-methylmalonyl-CoA Caldanaerobacter subterraneus subsp. tengcongensis
-
 (S)-methylmalonyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus subsp. tengcongensis Q8RCQ6
-
-

Purification (Commentary)

Purification (Comment) Organism
lysate is clarified by centrifugation and purified by passage through a nickel-affinity column, and a further purification step is performed by gel filtration Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-methylmalonyl-CoA
-
 Caldanaerobacter subterraneus subsp. tengcongensis (S)-methylmalonyl-CoA
-
 r

Subunits

Subunits Comment Organism
dimer in solution Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

Synonyms Comment Organism
methylmalonyl-CoA epimerase
-
 Caldanaerobacter subterraneus subsp. tengcongensis
MMCE
-
 Caldanaerobacter subterraneus subsp. tengcongensis
TTE0360
-
 Caldanaerobacter subterraneus subsp. tengcongensis

General Information

General Information Comment Organism
physiological function second step in the pathway responsible for the degradation of branched amino acids and odd chain fatty acids, MMCE participated in autotrophic CO2 fixation via the 3-hydroxypropionate pathway, in propionate fermentation, the regeneration of glyoxylate and in the biosynthesis of polyketide antibiotics Caldanaerobacter subterraneus subsp. tengcongensis