Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Rattus norvegicus | |
Cu2+ | - |
Rattus norvegicus | |
EDTA | reactivation by Co2+ | Rattus norvegicus | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | increases activity | Rattus norvegicus | |
Co3+ | lower level of activation than Co2+ at higher concentrations | Rattus norvegicus | |
Fe2+ | lower level of activation than Co2+ at higher concentrations | Rattus norvegicus | |
Mn2+ | lower level of activation than Co2+ at higher concentrations | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
32000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8400 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-Methyl-3-oxopropanoyl-CoA | - |
Rattus norvegicus | (S)-2-Methyl-3-oxopropanoyl-CoA | - |
? | |
DL-methylmalonyl-CoA | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4170 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 50% of maximal activity at pH 5.5 and 8.5 | Rattus norvegicus |