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Literature summary for 5.1.3.B12 extracted from
- Immobilization on graphene oxide improves the thermal stability and bioconversion efficiency of D-psicose 3-epimerase for rare sugar production (2017), Enzyme Microb. Technol., 107, 49-56 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | D-psicose (D-ribo-2-hexulose or D-allulose), a C3 epimer of D-fructose and considered as a rare sugar. It is regarded as a low calorie sweetener, an inhibitor of hepatic lipogenic enzymes, an activator of abdominal lipolysis and intestinal alpha-glycosidase enzymes D-psicose reduces the hyperglycemia, obesity, and hyperlipidemia and decrease the blood glucose level in type-2 diabetes | Agrobacterium tumefaciens |
| nutrition | D-psicose (D-ribo-2-hexulose or D-allulose), a C3 epimer of D-fructose and considered as a rare sugar. It is regarded as a low calorie sweetener, an inhibitor of hepatic lipogenic enzymes, an activator of abdominal lipolysis and intestinal alpha-glycosidase enzymes D-psicose reduces the hyperglycemia, obesity, and hyperlipidemia and decrease the blood glucose level in type-2 diabetes | Agrobacterium tumefaciens |
| synthesis | the enzyme can be used for synthesis of D-psicose (D-ribo-2-hexulose or D-allulose), a C3 epimer of D-fructose and considered as a rare sugar | Agrobacterium tumefaciens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene agtu, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of Agrobacterium tumefaciens DPEase (agtu-DPEase) on graphene oxide particles (GO-agtu-DPEase). Immobilization on graphene oxide improves the thermal stability and bioconversion efficiency of D-psicose 3-epimerase for rare sugar production. Graphene oxide immobilized agtu-DPEase (GO-agtu-DPEase) shows optima at pH 7.5 and 60°C. Significant improvement in thermal stability is observed with half-life of 720 min at 60°C while unbound (agtu) DPEase is stable for 3.99 min at 60°C. At equilibrium, the bioconversion efficiency is accounted 40:60 (D-psicose: D-fructose) for graphene oxide-immobilized DPEase which is higher than for the free agtu-DPEase (32:68). Graphene oxide immobilized DPEase shows bioconversion efficiency up to 10 cycles of reusability | Agrobacterium tumefaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose | Agrobacterium tumefaciens | - |
D-psicose | - |
r |  |
| D-fructose | Agrobacterium tumefaciens MTCC 609 / C58 / ATCC 33970 | - |
D-psicose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | A9CH28 | - |
- |
| Agrobacterium tumefaciens MTCC 609 / C58 / ATCC 33970 | A9CH28 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme 1.59fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | Agrobacterium tumefaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 66.89 | - |
purified recombinant enzyme, pH 7.5, 60°C | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose | - |
Agrobacterium tumefaciens | D-psicose | - |
r | |
| D-fructose | - |
Agrobacterium tumefaciens MTCC 609 / C58 / ATCC 33970 | D-psicose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, recombinant His-tagged enzyme, SDS-PAGE | Agrobacterium tumefaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| agtu | - |
Agrobacterium tumefaciens |
| DPEase | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
free enzyme DPEase | Agrobacterium tumefaciens |
| 60 | - |
recombinant graphene oxide-immobilized enzyme DPEase | Agrobacterium tumefaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
graphene oxide-immobilized enzyme DPEase has a half-life of 720 min, while unbound (agtu) DPEase is stable for 3.99 min | Agrobacterium tumefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
recombinant graphene oxide-immobilized enzyme DPEase | Agrobacterium tumefaciens |
| 8 | - |
free enzyme DPEase | Agrobacterium tumefaciens |
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