Application | Comment | Organism |
---|---|---|
synthesis | the enzyme from Agrobacterium tumefaciens can be used for production of D-psicose in a coexpression system with xylose isomerase gene from Escherichia coli. Method optimization and evaluation, overview | Agrobacterium tumefaciens |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis of the D-psicose 3-epimerase gene, recombinant expression in Escherichia coli strain BL21(DE3), co-expression with xylose isomerase gene from Escherichia coli strain MG1665 from plasmid pET-28a-DPE-xylA | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | for production of D-psicose from D-glucose in an enzymatic process, the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively. Co2+ is strictly required. Method optimization and evaluation, overview | Agrobacterium tumefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | highly activating, addition of Mg2+ to media containing Co2+ can further improve the conversion rate by 1.24fold, while Mg2+ alone is not activating | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | Agrobacterium tumefaciens | - |
D-psicose | - |
r | |
D-fructose | Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970 | - |
D-psicose | - |
r | |
additional information | Agrobacterium tumefaciens | the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively | ? | - |
? | |
additional information | Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970 | the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | A9CH28 | - |
- |
Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970 | A9CH28 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Agrobacterium tumefaciens | D-psicose | - |
r | |
D-fructose | - |
Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970 | D-psicose | - |
r | |
additional information | the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively | Agrobacterium tumefaciens | ? | - |
? | |
additional information | the xylose isomerase gene from Escherichia coli strain MG1665 and the D-psicose 3-epimerase gene from Agrobacterium tumefaciens CGMCC 1.1488 are coexpressed in Escherichia coli strain BL21(DE3). After 24 h incubation with the dual enzyme system at 40°C, the sugar conversion ratio from D-glucose to D-psicose reaches 10%. The optimal conditions are 50°C, pH 7.5 with Co2+ and Mg2+. The D-psicose yields from sugarcane bagasse and microalgae hydrolysate are 1.42 and 1.69 g/L, respectively | Agrobacterium tumefaciens CGMCC 1.1488 / C58 / ATCC 33970 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DPEase | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Agrobacterium tumefaciens |