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Literature summary for 5.1.3.7 extracted from
- Purification and some properties of UDP-N-acetylglucosamine 4-epimerase from Bacillus subtilis (1985), Agric. Biol. Chem., 49, 603-609.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Bacillus subtilis |  |
| glucosamine | competitive with UDP-N-acetylglucosamine | Bacillus subtilis | |
| HgCl2 | - |
Bacillus subtilis | |
| iodoacetate | - |
Bacillus subtilis | |
| NEM | - |
Bacillus subtilis | |
| PCMB | - |
Bacillus subtilis | |
| Zn2+ | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.4 | - |
UDP-N-acetylglucosamine | - |
Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates | Bacillus subtilis | |
| Co2+ | stimulates | Bacillus subtilis | |
| Mg2+ | stimulates | Bacillus subtilis | |
| Mn2+ | stimulates | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 565 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine | - |
Bacillus subtilis | UDP-N-acetyl-D-galactosamine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | without NAD+ | Bacillus subtilis |
| 40 | 45 | in presence of 1 mM NAD+ | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 7.5, 15 min, 65% loss of activity in presence of 1 mM NAD+, in absence of NAD+ complete loss of activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | 4°C, stable | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | highly stimulated by small amounts, Km: 0.13 mM | Bacillus subtilis | |
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