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Literature summary for 5.1.3.6 extracted from

  • Yin, S.; Sun, Y.J.; Liu, M.; Li, L.N.; Kong, J.Q.
    cDNA isolation and functional characterization of UDP-D-glucuronic acid 4-epimerase family from Ornithogalum caudatum (2016), Molecules, 21, 1505 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene UGlcAE3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time quantitative PCR enzyme expression analysis, recombinant expression in Pichia pastoris strain GS115 in the microsomal fraction. The recombinant OcUGlcAE3 can form a microsomal protein by the N-terminal membrane anchor sequence, or can be secrete into the extracellular space through the guidance of the signal peptide. OcUGlcAE3 can be expressed in Escherichia coli strain Transetta (DE3) only as truncated enzyme with deletion of a putative transmembrane domain of 128 aa in the N-terminus Albuca bracteata

Protein Variants

Protein Variants Comment Organism
additional information deletion of a putative transmembrane domain of 128 aa in the N-terminus, allowing the recombinant expression of a soluble enzyme. Deletion of the conversed GxxGxxG motif in turn results in the activity loss of OcUGlcAE3 Albuca bracteata

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Albuca bracteata 16020
-
microsome
-
Albuca bracteata
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucuronate Albuca bracteata
-
UDP-D-galacturonate
-
r

Organism

Organism UniProt Comment Textmining
Albuca bracteata A0A1J0KK43 i.e. Albuca bracteata
-

Source Tissue

Source Tissue Comment Organism Textmining
bulb sterile, moderate activity Albuca bracteata
-
leaf low activity Albuca bracteata
-
additional information negligible activity in non-sterile bulbs and flowers Albuca bracteata
-
root UGlcAE3 expression is root-specific, high activity Albuca bracteata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucuronate
-
Albuca bracteata UDP-D-galacturonate
-
r

Subunits

Subunits Comment Organism
? x * 50579, sequence calculation Albuca bracteata

Synonyms

Synonyms Comment Organism
OcUGlcAE3
-
Albuca bracteata
UDP-D-glucuronic acid 4-epimerase
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Albuca bracteata
UGlcAE
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Albuca bracteata
UGlcAE3
-
Albuca bracteata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
optimum 1 Albuca bracteata
42
-
optimum 2 Albuca bracteata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
60 activity range, M-type temperature profile, overview Albuca bracteata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 optimum 1 Albuca bracteata
10
-
optimum 2 Albuca bracteata

pH Range

pH Minimum pH Maximum Comment Organism
4 11 activity range, enzyme activity increases gradually with the increase of pH. When pH reaches the range of pH 8-8.5, the enzyme displays the highest activity. With the further increase of pH to about pH 9, the activity of the enzyme decreases slightly. At pH 9-10, the activity of OcUGlcAE3 increases gradually, and reaches the highest activity again at pH value of 10. Above the pH 10.0, the activity decreases, 80% of maximal activity at pH 12.0. M-type pH profile Albuca bracteata

Cofactor

Cofactor Comment Organism Structure
additional information recombinant OcUGlcAE3 does not require exogenous NAD+, suggesting the recombinant OcUGlcAE3 contains tightly bound NAD+ Albuca bracteata

pI Value

Organism Comment pI Value Maximum pI Value
Albuca bracteata sequence calculation
-
9.77

Expression

Organism Comment Expression
Albuca bracteata OcUGlcAE3 is proposed to be environmentally induced up

General Information

General Information Comment Organism
evolution the enzyme is a member of the UGlcAE family, a subfamily of the the SDR enzyme superfamily. The only three family members are OcUGlcAE1, OcUGlcAE2, and OcUGlcAE3, isolated from Ornithogalum caudatum. Phylogenetic relationships of OcUGlcAE with other UGlcAE, UGE (UDP-D-glucose 4-epimerase) and UXE (UDP-D-xylose 4-epimerase) enzymes are analyzed Albuca bracteata
malfunction deletion of the conversed GxxGxxG motif result in the activity loss of OcUGlcAE3 Albuca bracteata
additional information the N-terminus of UGlcAEs plays important roles in their soluble expression and 4-epimerase activity Albuca bracteata
physiological function UDP-D-glucuronic acid 4-epimerase OcUGlcAE3 is involved in the biosynthesis of root polysaccharides Albuca bracteata